BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27040

Title: NMR resonance assignments of a hypoallergenic isoform of the major birch pollen allergen Bet v 1   PubMed: 28587205

Deposition date: 2017-03-03 Original release date: 2017-08-10

Authors: Ahammer, Linda; Grutsch, Sarina; Wallner, Michael; Ferreira, Fatima; Tollinger, Martin

Citation: Grutsch, Sarina; Fuchs, Julian; Ahammer, Linda; Kamenik, Anna; Liedl, Klaus; Tollinger, Martin. "Conformational Flexibility Differentiates Naturally Occurring Bet v 1 Isoforms"  Int. J. Mol. Sci. 18, E1192-E1192 (2017).

Assembly members:
Bet_v_1.0102, polymer, 159 residues, 17417.69 Da.

Natural source:   Common Name: European white birch   Taxonomy ID: 3505   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Betula verrucosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Bet_v_1.0102: GVFNYEIETTSVIPAARLFK AFILDGDNLVPKVAPQAISS VENIEGNGGPGTIKKINFPE GFPFKYVKDRVDEVDHTNFK YNYSVIEGGPVGDTLEKISN EIKIVATPDGGCVLKISNKY HTKGNHEVKAEQVKASKEMG ETLLRAVESYLLAHSDAYN

Data sets:
Data typeCount
13C chemical shifts620
15N chemical shifts152
1H chemical shifts985

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bet v 1.0102, subunit 11
2Bet v 1.0102, subunit 21

Entities:

Entity 1, Bet v 1.0102, subunit 1 159 residues - 17417.69 Da.

1   GLYVALPHEASNTYRGLUILEGLUTHRTHR
2   SERVALILEPROALAALAARGLEUPHELYS
3   ALAPHEILELEUASPGLYASPASNLEUVAL
4   PROLYSVALALAPROGLNALAILESERSER
5   VALGLUASNILEGLUGLYASNGLYGLYPRO
6   GLYTHRILELYSLYSILEASNPHEPROGLU
7   GLYPHEPROPHELYSTYRVALLYSASPARG
8   VALASPGLUVALASPHISTHRASNPHELYS
9   TYRASNTYRSERVALILEGLUGLYGLYPRO
10   VALGLYASPTHRLEUGLULYSILESERASN
11   GLUILELYSILEVALALATHRPROASPGLY
12   GLYCYSVALLEULYSILESERASNLYSTYR
13   HISTHRLYSGLYASNHISGLUVALLYSALA
14   GLUGLNVALLYSALASERLYSGLUMETGLY
15   GLUTHRLEULEUARGALAVALGLUSERTYR
16   LEULEUALAHISSERASPALATYRASN

Samples:

sample_1: Bet v 1.0102, [U-99% 13C; U-99% 15N], 0.8 mM; sodium phosphate 10 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCCO)NH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

  • Varian DD2 500 MHz

Related Database Links:

UNP P43177

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts