BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27011

Title: Relaxation data for sigma1.1 from Bacillus subtilis   PubMed: 28539362

Authors: Zachrdla, Milan; Padrta, Petr; Rabatinova, Alzbeta; Sanderova, Hana; Barvik, Ivan; Krasny, Libor; Zidek, Lukas

Citation: Zachrdla, Milan; Padrta, Petr; Rabatinova, Alzbeta; Sanderova, Hana; Barvik, Ivan; Krasny, Libor; Zidek, Lukas. "Solution Structure of Domain 1.1 of the sigma(A) Factor from Bacillus subtilis is Preformed for Binding to the RNA Polymerase Core"  J. Biol. Chem. 292, 11610-11617 (2017).

Assembly members:
sigma1.1, polymer, 79 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
sigma1.1: ADKQTHETELTFDQVKEQLT ESGKKRGVLTYEEIAERMSS FEIESDQMDEYYEFLGEQGV ELISENEETEDLEHHHHHH

Data typeCount
T1 relaxation values204
T2 relaxation values206
heteronuclear NOE values204
order parameters76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1domain1.11

Entities:

Entity 1, domain1.1 79 residues - Formula weight is not available

1   ALAASPLYSGLNTHRHISGLUTHRGLULEU
2   THRPHEASPGLNVALLYSGLUGLNLEUTHR
3   GLUSERGLYLYSLYSARGGLYVALLEUTHR
4   TYRGLUGLUILEALAGLUARGMETSERSER
5   PHEGLUILEGLUSERASPGLNMETASPGLU
6   TYRTYRGLUPHELEUGLYGLUGLNGLYVAL
7   GLULEUILESERGLUASNGLUGLUTHRGLU
8   ASPLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: sigma1.1, [U-100% 15N], 0.6 mM; sodium chloride 10 mM; sodium azide 6 mM; phosphate buffer 20 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.6; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
2D R1 15N,1H correlationsample_1isotropicsample_conditions_1
2D R2 15N,1H correlationsample_1isotropicsample_conditions_1
2D NOE 15N,1H correlationsample_1isotropicsample_conditions_1
2D 15N1H correlation - transverse cross-correlated cross-relaxation (symmetrical reconversion)sample_1isotropicsample_conditions_1
2D 15N1H correlation - longitudinal cross-correlated cross-relaxation (symmetrical reconversion)sample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

Relax, d'Auvergne - data analysis

Octave, Eaton - data analysis

NMR spectrometers:

  • Bruker Avance 950 MHz
  • Bruker Avance 850 MHz
  • Bruker Avance 600 MHz

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