BMRB Entry 26938

Title:
Iminoproton chemical shifts of ASH1 E3 28mer RNA stem-loop
Deposition date:
2016-11-09
Original release date:
2016-12-29
Authors:
Schlundt, Andreas; Sattler, Michael; Edelmann, Franziska; Niessing, Dierk
Citation:

Citation: Edelmann, Franziska; Schlundt, Andreas; Heym, Roland; Jenner, Andreas; Niedner-Boblenz, Annika; Syed, Muhammad; Paillart, Jean-Christophe; Stehle, Ralf; Janowski, Robert; Sattler, Michael; Jansen, Ralf-Peter; Niessing, Dierk. "Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex"  Nat. Struct. Biol. 24, 152-161 (2017).
PubMed: 28092367

Assembly members:

Assembly members:
ASH1_E3_28mer, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: Saccharomyces cerevisiae   Vector: PCR product

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASH1_E3_28mer: GAUAACUGAAUCGAAAGACA UUAUCACG

Data sets:
Data typeCount
1H chemical shifts10

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASH1-mRNA E3 28mer stem-loop1

Entities:

Entity 1, ASH1-mRNA E3 28mer stem-loop 28 residues - Formula weight is not available

Sequence comprises three fragments corresponding to A, B and C in the CS files. A (1774-1785) and B (1803-1814) are strands of the wt stem sequence and C is an artificial loop (GAAA).

1   GAUAACUGAA
2   UCGAAAGACA
3   UUAUCACG

Samples:

sample_1: ASH1 E3 28mer 172 uM; HEPES 20 mM; sodium chloride 150 mM; magnesium chloride 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin, CCPN - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance 900 MHz