BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26884

Title: Backbone assignment of Rhea (talin) F0.   PubMed: 30446511

Deposition date: 2016-09-01 Original release date: 2018-11-26

Authors: Castle, Will; Tanentzapf, Guy; Goult, Ben

Citation: Camp, D; Haage, A; Solianova, V; Castle, W; Xu, Q; Lostchuck, E; Goult, B; Tanentzapf, G. "Direct binding of Talin to Rap1 is required for cell-ECM adhesion in Drosophila"  J. Cell. Sci. 131, 225144-225144 (2018).

Assembly members:
Rhea, polymer, 89 residues, 10243.5 Da.

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Rhea: SMMSTLSLRIQLEGGRVTKT IQFQPNTTVFDACKVIRDKF AEAVQGQPSEYGLFISDEQN QQGVWLEPGRTLGYYILHNQ DTLEYRRKT

Data sets:
Data typeCount
13C chemical shifts245
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rhea F01

Entities:

Entity 1, rhea F0 89 residues - 10243.5 Da.

Residues 1-2 represent a non-native affinity tag

1   SERMETMETSERTHRLEUSERLEUARGILE
2   GLNLEUGLUGLYGLYARGVALTHRLYSTHR
3   ILEGLNPHEGLNPROASNTHRTHRVALPHE
4   ASPALACYSLYSVALILEARGASPLYSPHE
5   ALAGLUALAVALGLNGLYGLNPROSERGLU
6   TYRGLYLEUPHEILESERASPGLUGLNASN
7   GLNGLNGLYVALTRPLEUGLUPROGLYARG
8   THRLEUGLYTYRTYRILELEUHISASNGLN
9   ASPTHRLEUGLUTYRARGARGLYSTHR

Samples:

15N_labelled: Rhea, [U-100% 15N], 100 ± 1 uM; D2O, [U-100% 2H], 5 ± 0.1 %; sodium phosphate 20 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; DTT 2 ± 0.1 mM

double_labelled: Rhea, [U-100% 13C; U-100% 15N], 500 ± 1 uM; D2O, [U-100% 2H], 5 ± 0.1 %; sodium phosphate 20 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; DTT 2 ± 0.1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_labelledisotropicsample_conditions_1
3D CBCA(CO)NHdouble_labelledisotropicsample_conditions_1
3D HNCOdouble_labelledisotropicsample_conditions_1
3D HNCAdouble_labelledisotropicsample_conditions_1
3D HNCACBdouble_labelledisotropicsample_conditions_1
3D HN(CO)CAdouble_labelledisotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CCPNMR_Analysis v2.15, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts