BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26854

Title: AsLOV2 (Avena sativa phototropin 1, LOV2 domain)   PubMed: 12970567

Deposition date: 2016-07-14 Original release date: 2016-08-11

Authors: Gardner, Kevin

Citation: Harper, Shannon; Neil, Lori; Gardner, Kevin. "Structural basis of a phototropin light switch"  Science 301, 1541-1544 (2003).

Assembly members:
AsLOV2, polymer, 161 residues, Formula weight is not available
FLAVIN MONONUCLEOTIDE, non-polymer, 456.344 Da.

Natural source:   Common Name: oat   Taxonomy ID: 4498   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Avena sativa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
AsLOV2: GEFLATTLERIEKNFVITDP RLPDNPIIFASDSFLQLTEY SREEILGRNCRFLQGPETDR ATVRKIRDAIDNQTEVTVQL INYTKSGKKFWNLFHLQPMR DQKGDVQYFIGVQLDGTEHV RDAAEREGVMLIKKTAENID EAAKELPDANLRPEDLWANH G

Data sets:
Data typeCount
13C chemical shifts644
15N chemical shifts166
1H chemical shifts987

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AsLOV2 protein1
2AsLOV2 FMN2

Entities:

Entity 1, AsLOV2 protein 161 residues - Formula weight is not available

residues 1-3 (GEF) and residue 161 (G) are vector-derived residues 4-160 correspond to residues 404-560 of A. sativa phototropin 1

1   GLYGLUPHELEUALATHRTHRLEUGLUARG
2   ILEGLULYSASNPHEVALILETHRASPPRO
3   ARGLEUPROASPASNPROILEILEPHEALA
4   SERASPSERPHELEUGLNLEUTHRGLUTYR
5   SERARGGLUGLUILELEUGLYARGASNCYS
6   ARGPHELEUGLNGLYPROGLUTHRASPARG
7   ALATHRVALARGLYSILEARGASPALAILE
8   ASPASNGLNTHRGLUVALTHRVALGLNLEU
9   ILEASNTYRTHRLYSSERGLYLYSLYSPHE
10   TRPASNLEUPHEHISLEUGLNPROMETARG
11   ASPGLNLYSGLYASPVALGLNTYRPHEILE
12   GLYVALGLNLEUASPGLYTHRGLUHISVAL
13   ARGASPALAALAGLUARGGLUGLYVALMET
14   LEUILELYSLYSTHRALAGLUASNILEASP
15   GLUALAALALYSGLULEUPROASPALAASN
16   LEUARGPROGLUASPLEUTRPALAASNHIS
17   GLY

Entity 2, AsLOV2 FMN - C17 H21 N4 O9 P - 456.344 Da.

1   FMN

Samples:

sample_1: AsLOV2, [U-100% 13C; U-100% 15N], 0.1 – 0.5 mM; sodium phosphate 50 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

GB AAC05083.1

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts