BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26788

Title: 1H, 13C and 15N backbone resonance assignments and 15N relaxation data of the PDZ tandem of Whirlin   PubMed: 27394726

Authors: Delhommel, Florent; Wolff, Nicolas; Cordier, Florence

Citation: Delhommel, Florent; Wolff, Nicolas; Cordier, Florence. "1H, 13C and 15N backbone resonance assignments and dynamic properties of the PDZ tandem of Whirlin"  Biomol. NMR Assign. 10, 361-365 (2016).

Assembly members:
Whirlin_PDZ1-PDZ2, polymer, 253 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Whirlin_PDZ1-PDZ2: GAMGSGEVRLVSLRRAKAHE GLGFSIRGGSEHGVGIYVSL VEPGSLAEKEGLRVGDQILR VNDKSLARVTHAEAVKALKG SKKLVLSVYSAGRIPGGYVT NHIYTWVDPQGRSTSPPSSL PQPHGSTLRQREDDRRSTLH LLQSGDEKKVNLVLGDGRSL GLTIRGGAEYGLGIYITGVD PGSEAESSGLKVGDQILEVN GRSFLNILHDEAVKLLKSSR HLILTVKDVGRLPHARTTVD QTKWIASSRIGES

Data sets:
Data typeCount
13C chemical shifts652
15N chemical shifts214
1H chemical shifts214
T1 relaxation values173
T2 relaxation values181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Whirlin PDZ1-PDZ21

Entities:

Entity 1, Whirlin PDZ1-PDZ2 253 residues - Formula weight is not available

1   GLYALAMETGLYSERGLYGLUVALARGLEU
2   VALSERLEUARGARGALALYSALAHISGLU
3   GLYLEUGLYPHESERILEARGGLYGLYSER
4   GLUHISGLYVALGLYILETYRVALSERLEU
5   VALGLUPROGLYSERLEUALAGLULYSGLU
6   GLYLEUARGVALGLYASPGLNILELEUARG
7   VALASNASPLYSSERLEUALAARGVALTHR
8   HISALAGLUALAVALLYSALALEULYSGLY
9   SERLYSLYSLEUVALLEUSERVALTYRSER
10   ALAGLYARGILEPROGLYGLYTYRVALTHR
11   ASNHISILETYRTHRTRPVALASPPROGLN
12   GLYARGSERTHRSERPROPROSERSERLEU
13   PROGLNPROHISGLYSERTHRLEUARGGLN
14   ARGGLUASPASPARGARGSERTHRLEUHIS
15   LEULEUGLNSERGLYASPGLULYSLYSVAL
16   ASNLEUVALLEUGLYASPGLYARGSERLEU
17   GLYLEUTHRILEARGGLYGLYALAGLUTYR
18   GLYLEUGLYILETYRILETHRGLYVALASP
19   PROGLYSERGLUALAGLUSERSERGLYLEU
20   LYSVALGLYASPGLNILELEUGLUVALASN
21   GLYARGSERPHELEUASNILELEUHISASP
22   GLUALAVALLYSLEULEULYSSERSERARG
23   HISLEUILELEUTHRVALLYSASPVALGLY
24   ARGLEUPROHISALAARGTHRTHRVALASP
25   GLNTHRLYSTRPILEALASERSERARGILE
26   GLYGLUSER

Samples:

sample_1: Whirlin PDZ1-PDZ2, [U-100% 15N], 175 uM; H2O 90%; D2O 10%; NaCl 150 mM; Tris-HCl 50 mM

sample_2: Whirlin PDZ1-PDZ2, [U-100% 13C; U-100% 15N; U-80% 2H], 600 uM; H2O 90%; D2O 10%; NaCl 150 mM; Tris-HCl 50 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D HN(CA)COsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
15N T1 experimentsample_1isotropicsample_conditions_1
15N T2 experimentsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

CCPNMR_Analysis, CCPN - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 900 MHz

Related Database Links: