BMRB Entry 26760

Title:
1H, 13C and 15N NMR assignments of an unusual Ca2+-binding protein from Entamoeba histolytica in its apo form
Deposition date:
2016-03-18
Original release date:
2017-03-02
Authors:
Verma, Deepshikha; Chary, Kandala
Citation:

Citation: Verma, Deepshikha; Sakuntala, Mutyala; Murmu, Aruna; Bhattacharya, Alok; Chary, Kandala. "(1)H, (13)C and (15)N NMR assignments of an unusual Ca(2+)-binding protein from Entamoeba histolytica in its apo form."  Biomol. NMR Assign. 11, 63-67 (2017).
PubMed: 27990612

Assembly members:

Assembly members:
apo-EhCaBP6, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Entamoeba histolytica   Taxonomy ID: 5759   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Entamoeba histolytica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET21C

Data sets:
Data typeCount
13C chemical shifts345
15N chemical shifts123
1H chemical shifts763

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-EhCaBP61

Entities:

Entity 1, apo-EhCaBP6 156 residues - Formula weight is not available

1   METSERMETGLUILEGLUALAPROASNALA
2   ASNTHRGLNLYSILEARGASPCYSPHEASN
3   PHETYRASPARGASPTYRASPGLYLYSILE
4   ASPVALLYSGLNLEUGLYTHRLEUILEARG
5   SERLEUGLYCYSALAPROTHRGLUASPGLU
6   VALASNSERTYRILELYSGLUPHEALAILE
7   GLUGLYGLUTHRPHEGLNILEGLUGLNPHE
8   GLULEUILEMETGLUARGGLUGLNSERLYS
9   PROASPTHRARGGLUILELYSLEUARGLYS
10   ALAPHEGLUVALPHEASPGLNASPLYSASP
11   GLYLYSILELYSALASERASPLEUALAHIS
12   ASNLEUTHRTHRVALGLYASPLYSMETTHR
13   LYSGLUGLUVALGLULYSVALPHESERILE
14   LEUGLYILETHRMETGLUSERASPILEASP
15   LEUALATHRPHELEULYSLEUVALALALEU
16   HISHISHISHISHISHIS

Samples:

sample_1: apo-EhCaBP6, [U-100% 13C; U-100% 15N], 1 mM; TRIS 50 mM; sodium chloride 50 mM; beta-mercaptoethanol 5 mM

sample_2: apo-EhCaBP6, [U-100% 13C; U-100% 15N], 1 mM; TRIS 50 mM; sodium chloride 50 mM; beta-mercaptoethanol 5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks