BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26723

Title: NMR chemical shift assignments for the C-terminal domain of Tetrahymena Tcb2 in the absence of calcium   PubMed: 27155947

Authors: Kilpatrick, Adina; Gurrola, Theodore; Fowler, C. Andrew

Citation: Kilpatrick, Adina; Gurrola, Theodore; Sterner, Robert; Sleister, Heidi; Honts, Jerry; Fowler, C. Andrew. "Backbone and side-chain chemical shift assignments for the C-terminal domain of Tcb2, a cytoskeletal calcium-binding protein from Tetrahymena thermophila"  Biomol. NMR Assign. ., .-. (2016).

Assembly members:
Tcb2-C, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: Tetrahymena thermophila   Taxonomy ID: 5911   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Tetrahymena thermophila

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Tcb2-C: SSKPKYNPEVEAKLDVARRL FKRYDKDGSGQLQDDEIAGL LKDTYAEMGMSNFTPTKEDV KIWLQMADTNSDGSVSLEEY EDLIIKSLQKAGIRVEKQSL VF

Data sets:
Data typeCount
13C chemical shifts450
15N chemical shifts101
1H chemical shifts677
T1 relaxation values87
T2 relaxation values84
heteronuclear NOE values87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tcb2-C1

Entities:

Entity 1, Tcb2-C 102 residues - Formula weight is not available

C-terminal domain of Tetrahymena calcium-binding protein 2 (Tcb2)

1   SERSERLYSPROLYSTYRASNPROGLUVAL
2   GLUALALYSLEUASPVALALAARGARGLEU
3   PHELYSARGTYRASPLYSASPGLYSERGLY
4   GLNLEUGLNASPASPGLUILEALAGLYLEU
5   LEULYSASPTHRTYRALAGLUMETGLYMET
6   SERASNPHETHRPROTHRLYSGLUASPVAL
7   LYSILETRPLEUGLNMETALAASPTHRASN
8   SERASPGLYSERVALSERLEUGLUGLUTYR
9   GLUASPLEUILEILELYSSERLEUGLNLYS
10   ALAGLYILEARGVALGLULYSGLNSERLEU
11   VALPHE

Samples:

sample_1: Tcb2-C, [U-98% 13C; U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM

sample_2: Tcb2-C, [U-98% 13C; U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM

sample_3: Tcb2-C, [U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM

sample_4: Tcb2-C 1.1 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
2D 1H-1H COSYsample_4isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_2isotropicsample_conditions_1
Heteronuclear NOE ratiosample_1isotropicsample_conditions_1
T1 experimentssample_1isotropicsample_conditions_1
T2 experimentssample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis, CCPN - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

NCBI P09226.2 XP_001007754.3