BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26714

Title: Relaxation and model-free data from human beta-2-microglobulin   PubMed: 18085410

Authors: Ballaschk, Martin; Uchanska-Ziegler, Barbara; Ziegler, Andreas; Diehl, Anne; Schmieder, Peter

Citation: d'Auvergne, Edward; Gooley, Paul. "Optimisation of NMR dynamic models I. Minimisation algorithms and their performance within the model-free and Brownian rotational diffusion spaces."  J. Biomol. NMR 40, 107-119 (2008).

Assembly members:
1lds-hkc_mol1, polymer, 100 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
1lds-hkc_mol1: MIQRTPKIQVYSRHPAENGK SNFLNCYVSGFHPSDIEVDL LKNGERIEKVEHSDLSFSKD WSFYLLYYTEFTPTEKDEYA CRVNHVTLSQPKIVKWDRDM

Data sets:
Data typeCount
General relaxation values248
chemical shift anisotropy values62
heteronuclear NOE values124
order parameters62
tensors62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1b2m1

Entities:

Entity 1, b2m 100 residues - Formula weight is not available

1   METILEGLNARGTHRPROLYSILEGLNVAL
2   TYRSERARGHISPROALAGLUASNGLYLYS
3   SERASNPHELEUASNCYSTYRVALSERGLY
4   PHEHISPROSERASPILEGLUVALASPLEU
5   LEULYSASNGLYGLUARGILEGLULYSVAL
6   GLUHISSERASPLEUSERPHESERLYSASP
7   TRPSERPHETYRLEULEUTYRTYRTHRGLU
8   PHETHRPROTHRGLULYSASPGLUTYRALA
9   CYSARGVALASNHISVALTHRLEUSERGLN
10   PROLYSILEVALLYSTRPASPARGASPMET

Samples:

sample_1: 1lds-hkc_mol1, [U-15N], 0.43 mM; D2O, [U-2H], 10%; H2O 90%; sodium chloride 150 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 0.160 M; pH: 7.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
600.133 MHz R1sample_1isotropicsample_conditions_1
600.133 MHz R2sample_1isotropicsample_conditions_1
600.133 MHz steady-state NOEsample_1isotropicsample_conditions_1
750.46 MHz R1sample_1isotropicsample_conditions_1
750.46 MHz R2sample_1isotropicsample_conditions_1
750.46 MHz steady-state NOEsample_1isotropicsample_conditions_1

Software:

relax vrepository checkout, The relax development team - data processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 750 MHz

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