BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26706

Title: Backbone 13C, and 15 N Chemical Shift Assignments for fibrillar Islet Amyloid Polypeptide (IAPP)   PubMed: 27607147

Authors: Weirich, Franziska; Heise, Henrike; Mirecka, Ewa; Gremer, Lothar; Hoyer, Wolfgang; Schiefer, Stephanie

Citation: Weirich, Franziska; Mirecka, Ewa; Schiefer, Stephanie; Gremer, Lothar; Hoyer, Wolfgang; Heise, Henrike. "Structural Characterization of Fibrils from Recombinant Human Islet Amyloid Polypeptide by Solid-State NMR: The Central FGAILS Segment Is Part of the beta-Sheet Core"  PLOS One 11, e0161243-e0161243 (2016).

Assembly members:
IAPP, polymer, 37 residues, 3906.3 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IAPP: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:
Data typeCount
13C chemical shifts109
15N chemical shifts37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1recombinant fibrillar hIAPP1

Entities:

Entity 1, recombinant fibrillar hIAPP 37 residues - 3906.3 Da.

Full length human IAPP.

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSERSER
3   ASNASNPHEGLYALAILELEUSERSERTHR
4   ASNVALGLYSERASNTHRTYR

Samples:

sample_1: IAPP, [U-100% 13C; U-100% 15N], 110 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C PDSDsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D NCaCBsample_1isotropicsample_conditions_1
3D NCaCXsample_1isotropicsample_conditions_1
1D INEPTsample_1isotropicsample_conditions_1
2D DQSQ SPC5_3sample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2, CCPN - chemical shift assignment

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Oxford AS600 600 MHz
  • Varian 600/54 Premium Shielded 600 MHz
  • Oxford 2.2K 800/63 800 MHz

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