BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26636

Title: 1H and 15N Chemical Shifts of Intrinsically Disordered Dehydrin ERD14 at Various Temperature and pH Values

Authors: Szalaine Agoston, Bianka

Citation: Kalmar, Lajos; Szalaine Agoston, Bianka; Magos, Zoltan; Felmery, Anna; Szabo, Beata; Tantos, Agnes; Murvai, Nikoletta; Csikos, Gyorgy; Vertomen, Didier; Hristova, Nevena; Kovacs, Denes; Lang, Andras; Buday, Laszlo; Han, Kyou-Hoon; Perczel, Andras; Tompa, Peter. "Interplay of structural disorder and induced folding in the chaperone activity of intrinsically disordered plant dehydrin ERD14 in vivo"  Cell ., .-..

Assembly members:
ERD14, polymer, 185 residues, 20786.3 Da.

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ERD14: MAEEIKNVPEQEVPKVATEE SSAEVTDRGLFDFLGKKKDE TKPEETPIASEFEQKVHISE PEPEVKHESLLEKLHRSDSS SSSSSEEEGSDGEKRKKKKE KKKPTTEVEVKEEEKKGFME KLKEKLPGHKKPEDGSAVAA APVVVPPPVEEAHPVEKKGI LEKIKEKLPGYHPKTTVEEE KKDKE

Data typeCount
15N chemical shifts916
1H chemical shifts916

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ERD141

Entities:

Entity 1, ERD14 185 residues - 20786.3 Da.

1   METALAGLUGLUILELYSASNVALPROGLU
2   GLNGLUVALPROLYSVALALATHRGLUGLU
3   SERSERALAGLUVALTHRASPARGGLYLEU
4   PHEASPPHELEUGLYLYSLYSLYSASPGLU
5   THRLYSPROGLUGLUTHRPROILEALASER
6   GLUPHEGLUGLNLYSVALHISILESERGLU
7   PROGLUPROGLUVALLYSHISGLUSERLEU
8   LEUGLULYSLEUHISARGSERASPSERSER
9   SERSERSERSERSERGLUGLUGLUGLYSER
10   ASPGLYGLULYSARGLYSLYSLYSLYSGLU
11   LYSLYSLYSPROTHRTHRGLUVALGLUVAL
12   LYSGLUGLUGLULYSLYSGLYPHEMETGLU
13   LYSLEULYSGLULYSLEUPROGLYHISLYS
14   LYSPROGLUASPGLYSERALAVALALAALA
15   ALAPROVALVALVALPROPROPROVALGLU
16   GLUALAHISPROVALGLULYSLYSGLYILE
17   LEUGLULYSILELYSGLULYSLEUPROGLY
18   TYRHISPROLYSTHRTHRVALGLUGLUGLU
19   LYSLYSASPLYSGLU

Samples:

sample_1: ERD14, [U-15N], 1 mM; DSS 1 mM; sodium phosphate 32 mM; potassium phosphate 22 mM; sodium chloride 8.6 mM; calcium chloride 0.1 mM; magnesium sulfate 1 mM; carbenicillin 50 mg/L; glucose.H2O 4 g/L; H2O 90%; D2O 10%

288K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 288 K

282.5K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 282.5 K

277K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 277 K

277K-6.94pH: pH: 6.94; pressure: 1 atm; temperature: 277 K

277K-7.29pH: pH: 7.29; pressure: 1 atm; temperature: 277 K

277K-7.42pH: pH: 7.42; pressure: 1 atm; temperature: 277 K

277K-7.57pH: pH: 7.57; pressure: 1 atm; temperature: 277 K

277K-7.70pH: pH: 7.70; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropic288K-6.58pH
2D 1H-15N HSQCsample_1isotropic282.5K-6.58pH
2D 1H-15N HSQCsample_1isotropic277K-6.58pH
2D 1H-15N HSQCsample_1isotropic277K-6.94pH
2D 1H-15N HSQCsample_1isotropic277K-7.29pH
2D 1H-15N HSQCsample_1isotropic277K-7.42pH
2D 1H-15N HSQCsample_1isotropic277K-7.57pH
2D 1H-15N HSQCsample_1isotropic277K-7.70pH

Software:

CARA v1.8.4.2, Rochus Keller - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

UNP P42763
BMRB 16876
DBJ BAA04569 BAE98985
GB AAF17644 AAG40050 AAG41486 AAK00404 AAK62649
REF NP_001185408 NP_177745
SP P42763