BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26626

Title: Backbone 1H, 13C and 15N resonance assignments for CaM-Fas-6-35 complex   PubMed: 26735300

Authors: Vlach, Jiri; Chang, Bliss; Samal, Alexandra; Fernandez, Timothy; Brooke, Dewey; Prevelige, Peter; Saad, Jamil

Citation: Chang, Bliss; Samal, Alexandra; Vlach, Jiri; Fernandez, Timothy; Brooke, Dewey; Prevelige, Peter; Saad, Jamil. "Identification of the Calmodulin-Binding Domains of Fas Death Receptor"  PLoS One 11, e0146493-e0146493 (2016).

Assembly members:
CaM, polymer, 148 residues, 16683.1669 Da.
Fas-6-35, polymer, 30 residues, 3256.7986 Da.

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CaM: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
Fas-6-35: DVDLSKYITTIAGVMTLSQV KGFVRKNGVN

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts146
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM1
2Fas-6-352

Entities:

Entity 1, CaM 148 residues - 16683.1669 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, Fas-6-35 30 residues - 3256.7986 Da.

This is a fragment of FAS death domain, FAS residues 226-255.

1   ASPVALASPLEUSERLYSTYRILETHRTHR
2   ILEALAGLYVALMETTHRLEUSERGLNVAL
3   LYSGLYPHEVALARGLYSASNGLYVALASN

Samples:

15N: CaM, [[U-95% 13C; U-95% 15N]], 0.5 mM; Fas-6-35 0.5 mM; H2O 90%; D2O 10%; Tris 50 mM; NaCl 100 mM; CaCl2 5 mM

13C15N: CaM, [[U-95% 13C; U-95% 15N]], 0.5 mM; Fas-6-35 0.5 mM; H2O 90%; D2O 10%; Tris 50 mM; NaCl 100 mM; CaCl2 5 mM

cond: ionic strength: 0.115 M; pH: 7.000; pressure: 1.000 atm; temperature: 308.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQC15Nisotropiccond
3D HNCA13C15Nisotropiccond
3D HNCACB13C15Nisotropiccond
HNcoCA (H[N[co[{CA|ca[C]}]]])13C15Nisotropiccond
HNcoCACB (H[N[co[{CA|ca[C]}]]])13C15Nisotropiccond
3D 1H-15N NOESY15Nisotropiccond

Software:

CcpNmr_Analysis v2.4, CCPN - data analysis

nmrDraw vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum analysis, Spectrum display

nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

nmrView vany, Johnson, One Moon Scientific - Spectrum analysis, Spectrum display

NMR spectrometers:

  • Bruker DMX 700 MHz

Related Database Links:

NCBI NP_000034.1