BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26614

Title: Solid-state NMR assignment of MxiH (serotype 5a) T3SS needles   PubMed: 26439285

Authors: Verasdonck, Joeri; Shen, Da-Kang; Treadgold, Alexander; Arthur, Christopher; Boeckmann, Anja; Meier, Beat; Blocker, Ariel

Citation: Verasdonck, Joeri; Shen, Da-Kang; Treadgold, Alexander; Arthur, Christopher; Boeckmann, Anja; Meier, Beat; Blocker, Ariel. "Reassessment of MxiH subunit orientation and fold within native Shigella T3SS needles using surface labelling and solid-state NMR"  J. Struct. Biol. 192, 441-448 (2015).

Assembly members:
MxiH, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: enterobacteria   Taxonomy ID: 623   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shigella flexneri

Experimental source:   Production method: purified from the natural source   Host organism: Shigella flexnier

Entity Sequences (FASTA):
MxiH: MSVTVPNDDWTLSSLSETFD DGTQTLQGELTLALDKLAKN PSNPQLLAEYQSKLSEYTLY RNAQSNTVKVIKDVDAAIIQ NFR

Data sets:
Data typeCount
13C chemical shifts378
15N chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MxiH1

Entities:

Entity 1, MxiH 83 residues - Formula weight is not available

1   METSERVALTHRVALPROASNASPASPTRP
2   THRLEUSERSERLEUSERGLUTHRPHEASP
3   ASPGLYTHRGLNTHRLEUGLNGLYGLULEU
4   THRLEUALALEUASPLYSLEUALALYSASN
5   PROSERASNPROGLNLEULEUALAGLUTYR
6   GLNSERLYSLEUSERGLUTYRTHRLEUTYR
7   ARGASNALAGLNSERASNTHRVALLYSVAL
8   ILELYSASPVALASPALAALAILEILEGLN
9   ASNPHEARG

Samples:

sample_1: MxiH, [U-100% 13C; U-100% 15N], 10 mg

sample_conditions_1: pH: 7.4; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 20ms DARRsample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
2D 200ms DARRsample_1isotropicsample_conditions_1
3D CCCsample_1isotropicsample_conditions_1
3D NCACBsample_1isotropicsample_conditions_1

Software:

CcpnNMR_Analysis v2.4, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 850 MHz

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