BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26550

Title: Sequence-specific solid-state NMR assignments of the mouse ASC PYRIN domain in its filament form   PubMed: 26464513

Authors: Ravotti, Francesco; Sborgi, Lorenzo; Cadalbert, Riccardo; Huber, Matthias; Hiller, Sebastian; Meier, Beat; Boeckmann, Anja

Citation: Sborgi, Lorenzo; Ravotti, Francesco; Dandey, Venkata; Dick, Mathias; Mazur, Adam; Reckel, Sina; Chami, Mohamed; Scherer, Sebastian; Huber, Matthias; Bockmann, Anja; Egelman, Edward; Stahlberg, Henning; Broz, Petr; Meier, Beat; Hiller, Sebastian. "Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy"  Proc. Natl. Acad. Sci. U. S. A. 112, 13237-13242 (2015).

Assembly members:
Mouse_ASC_PYD, polymer, 91 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Mouse_ASC_PYD: MGRARDAILDALENLSGDEL KKFKMKLLTVQLREGYGRIP RGALLQMDAIDLTDKLVSYY LESYGLELTMTVLRDMGLQE LAEQLQTTKEE

Data sets:
Data typeCount
13C chemical shifts395
15N chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mouse ASC PYD1

Entities:

Entity 1, Mouse ASC PYD 91 residues - Formula weight is not available

1   METGLYARGALAARGASPALAILELEUASP
2   ALALEUGLUASNLEUSERGLYASPGLULEU
3   LYSLYSPHELYSMETLYSLEULEUTHRVAL
4   GLNLEUARGGLUGLYTYRGLYARGILEPRO
5   ARGGLYALALEULEUGLNMETASPALAILE
6   ASPLEUTHRASPLYSLEUVALSERTYRTYR
7   LEUGLUSERTYRGLYLEUGLULEUTHRMET
8   THRVALLEUARGASPMETGLYLEUGLNGLU
9   LEUALAGLUGLNLEUGLNTHRTHRLYSGLU
10   GLU

Samples:

sample_1: Mouse ASC PYD, [U-100% 13C; U-100% 15N], 20 mg

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
NCACBsample_1isotropicsample_conditions_1
CANCOsample_1isotropicsample_conditions_1
CCCsample_1isotropicsample_conditions_1
NCAsample_1isotropicsample_conditions_1
NCOsample_1isotropicsample_conditions_1
20msDARRsample_1isotropicsample_conditions_1
N(CO)CACBsample_1isotropicsample_conditions_1
CAN(CO)CAsample_1isotropicsample_conditions_1
N(CA)CBCXsample_1isotropicsample_conditions_1
100msPDSDsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin, CCPN - collection, data analysis, processing

NMR spectrometers:

  • Bruker Avance 850 MHz

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