BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26533

Title: Targeting and Maturation of Erv1/ALR in the Mitochondrial Intermembrane Space   PubMed: 22296668

Authors: Kallergi, Emmanouela; Andreadaki, Maria; Kritsiligkou, Paraskevi; Katrakili, Nitsa; Pozidis, Charalambos; Tokatlidis, Kostas; Banci, Lucia; Bertini, Ivano; Cefaro, Chiara; Ciofi-Baffoni, Simone; Gajda, Karolina; Peruzzini, Riccardo

Citation: Kallergi, Emmanouela; Andreadaki, Maria; Kritsiligkou, Paraskevi; Katrakili, Nitsa; Pozidis, Charalambos; Tokatlidis, Kostas; Banci, Lucia; Bertini, Ivano; Cefaro, Chiara; Ciofi-Baffoni, Simone; Gajda, Karolina; Peruzzini, Riccardo. "Targeting and Maturation of Erv1/ALR in the Mitochondrial Intermembrane Space"  ACS Chem. Biol. 7, 707-714 (2012).

Assembly members:
sf-ALR, polymer, 125 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
sf-ALR: MRTQQKRDTKFREDCPPDRE ELGRHSWAVLHTLAAYYPDL PTPEQQQDMAQFIHLFSKFY PCEECAEDLRKRLARNHPDT RTRAAFTQWLCHLHNEVNRK LGKPDFDCSKVDERWRDGWK DGSCD

Data sets:
Data typeCount
heteronuclear NOE values43
T2 relaxation values43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E. coli-purified sf-ALR, chain 11
2E. coli-purified sf-ALR, chain 21

Entities:

Entity 1, E. coli-purified sf-ALR, chain 1 125 residues - Formula weight is not available

1   METARGTHRGLNGLNLYSARGASPTHRLYS
2   PHEARGGLUASPCYSPROPROASPARGGLU
3   GLULEUGLYARGHISSERTRPALAVALLEU
4   HISTHRLEUALAALATYRTYRPROASPLEU
5   PROTHRPROGLUGLNGLNGLNASPMETALA
6   GLNPHEILEHISLEUPHESERLYSPHETYR
7   PROCYSGLUGLUCYSALAGLUASPLEUARG
8   LYSARGLEUALAARGASNHISPROASPTHR
9   ARGTHRARGALAALAPHETHRGLNTRPLEU
10   CYSHISLEUHISASNGLUVALASNARGLYS
11   LEUGLYLYSPROASPPHEASPCYSSERLYS
12   VALASPGLUARGTRPARGASPGLYTRPLYS
13   ASPGLYSERCYSASP

Samples:

sample_1: E. coli-purified sf-ALR, [U-100% 15N], 0.5 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 308 K; pH: 7.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1isotropicsample_conditions_1
1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN_1.3, Bruker Biospin - processing

CARA, Keller and Wuthrich - data analysis

CSI, Wishart, D. S., and Sykes, B. D. - chemical shift index analysis

PECAN, Wishart, D. S., and Sykes, B. D. - chemical shift index analysis

NMR spectrometers:

  • Bruker NA 600 MHz

Related Database Links:

BMRB 18029 26515
PDB
DBJ BAI46852
EMBL CAB87993
GB AAA96390 AAD17327 AAD17328 AAD36986 AAG43494
REF NP_005253 XP_004057029 XP_007980253
SP P55789