BMRB Entry 26520

Title:
Studying Invisible Excited Protein States in Slow Exchange with a Major State Conformation
Deposition date:
2015-02-24
Original release date:
2015-03-09
Authors:
Vallurupalli, Pramodh; Bouvignies, Guillaume; Kay, Lewis
Citation:

Citation: Vallurupalli, Pramodh; Bouvignies, Guillaume; Kay, Lewis. "Studying Invisible Excited Protein States in Slow Exchange with a Major State Conformation"  J. Am. Chem. Soc. 134, 8148-8161 (2012).
PubMed: 22554188

Assembly members:

Assembly members:
A39G_FF, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSET

Entity Sequences (FASTA):

Data sets:
Data typeCount
T1 relaxation values65
T2 relaxation values130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A39G FF domain1

Entities:

Entity 1, A39G FF domain 71 residues - Formula weight is not available

1   GLUSERGLNPROALALYSLYSTHRTYRTHR
2   TRPASNTHRLYSGLUGLUALALYSGLNALA
3   PHELYSGLULEULEULYSGLULYSARGVAL
4   PROSERASNALASERTRPGLUGLNALAMET
5   LYSMETILEILEASNASPPROARGTYRSER
6   ALALEUALALYSLEUSERGLULYSLYSGLN
7   ALAPHEASNALATYRLYSVALGLNTHRGLU
8   LYS

Samples:

sample_1: A39G_FF, [U-100% 15N], 2 mM; sodium acetate 50 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 274 K; pH: 5.7; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HMQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - Visualization

CATIA, Bloch McConnell - data analysis

NMRpipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 17489 18010 19591
PDB
DBJ BAB15016 BAG60744
GB AAC27501 AAC27506 AAD39463 AAH11788 AAH29414
REF NP_001099950 NP_001231502 NP_060362 NP_061255 XP_001365479
SP O75400 Q9R1C7
TPG DAA32724
AlphaFold Q9R1C7 O75400