BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26519

Title: Studying Invisible Excited Protein States in Slow Exchange with a Major State Conformation   PubMed: 22554188

Authors: Vallurupalli, Pramodh; Bouvignies, Guillaume; Kay, Lewis

Citation: Vallurupalli, Pramodh; Bouvignies, Guillaume; Kay, Lewis. "Studying Invisible Excited Protein States in Slow Exchange with a Major State Conformation"  J. Am. Chem. Soc. 134, 8148-8161 (2012).

Assembly members:
Abp1p, polymer, 59 residues, Formula weight is not available
Ark1p, polymer, 17 residues, Formula weight is not available

Natural source:   Common Name: bakers yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Abp1p: APWATAEYDYDAAEDNELTF VENDKIINIEFVDDDWWLGE LEKDGSKGLFPSNYVSLGN
Ark1p: KKTKPTPPPKPSHLKPK

Data sets:
Data typeCount
T1 relaxation values56
T2 relaxation values112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abp1p1
2Ark1p2

Entities:

Entity 1, Abp1p 59 residues - Formula weight is not available

1   ALAPROTRPALATHRALAGLUTYRASPTYR
2   ASPALAALAGLUASPASNGLULEUTHRPHE
3   VALGLUASNASPLYSILEILEASNILEGLU
4   PHEVALASPASPASPTRPTRPLEUGLYGLU
5   LEUGLULYSASPGLYSERLYSGLYLEUPHE
6   PROSERASNTYRVALSERLEUGLYASN

Entity 2, Ark1p 17 residues - Formula weight is not available

1   LYSLYSTHRLYSPROTHRPROPROPROLYS
2   PROSERHISLEULYSPROLYS

Samples:

sample_1: Abp1p SH3 domain, [U-100% 2H; U-100% 15N], 1.5 mM; Ark1p peptide 1.46 mM; sodium phosphate 50 mM; NaCl 100 mM; EDTA 1 mM; NaN3 1 mM; H20 90%; D2O 10%

sample_2: Abp1p SH3 domain, [U-100% 2H; U-100% 15N], 1.5 mM; Ark1p peptide 1.5 mM; sodium phosphate 50 mM; NaCl 100 mM; EDTA 1 mM; NaN3 1 mM; H20 90%; D2O 10%

sample_conditions_1: temperature: 274 K; pH: 7.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC 1sample_1isotropicsample_conditions_1
2D 1H-15N HSQC 2sample_2isotropicsample_conditions_1
2D 1H-15N HMQC 1sample_1isotropicsample_conditions_1
2D 1H-15N HMQC 2sample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - Visualization

CATIA, Bloch McConnell - data analysis

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 16843 18054 18055 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 18078 25354
PDB
DBJ GAA21988
EMBL CAA36075 CAA42253 CAY78291
GB AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991
PRF 1603360A
REF NP_010012
SP P15891
TPG DAA07557