BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26514

Title: Temperature Dependence of Molecular Interactions Involved in Defining Stability of Glutamine Binding Protein and Its Complex with L-Glutamine   PubMed: 22206385

Deposition date: 2015-02-19 Original release date: 2015-03-02

Authors: Pistolesi, Sara; Tjandra, Nico

Citation: Pistolesi, Sara; Tjandra, Nico. "Temperature Dependence of Molecular Interactions Involved in Defining Stability of Glutamine Binding Protein and Its Complex with L-Glutamine"  Biochemistry 51, 643-652 (2012).

Assembly members:
GlnBP, polymer, 226 residues, Formula weight is not available
GLUTAMINE, non-polymer, 146.144 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GlnBP: ADKKLVVATDTAFVPFEFKQ GDKYVGFDVDLWAAIAKELK LDYELKPMDFSGIIPALQTK NVDLALAGITITDERKKAID FSDGYYKSGLLVMVKANNND VKSVKDLDGKVVAVKSGTGS VDYAKANIKTKDLRQFPNID NAYMELGTNRADAVLHDTPN ILYFIKTAGNGQFKAVGDSL EAQQYGIAFPKGSDELRDKV NGALKTLRENGTYNEIYKKW FGTEPK

Data typeCount
order parameters2676

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Glutamine binding protein1
2L-glutamine2

Entities:

Entity 1, Glutamine binding protein 226 residues - Formula weight is not available

1   ALAASPLYSLYSLEUVALVALALATHRASP
2   THRALAPHEVALPROPHEGLUPHELYSGLN
3   GLYASPLYSTYRVALGLYPHEASPVALASP
4   LEUTRPALAALAILEALALYSGLULEULYS
5   LEUASPTYRGLULEULYSPROMETASPPHE
6   SERGLYILEILEPROALALEUGLNTHRLYS
7   ASNVALASPLEUALALEUALAGLYILETHR
8   ILETHRASPGLUARGLYSLYSALAILEASP
9   PHESERASPGLYTYRTYRLYSSERGLYLEU
10   LEUVALMETVALLYSALAASNASNASNASP
11   VALLYSSERVALLYSASPLEUASPGLYLYS
12   VALVALALAVALLYSSERGLYTHRGLYSER
13   VALASPTYRALALYSALAASNILELYSTHR
14   LYSASPLEUARGGLNPHEPROASNILEASP
15   ASNALATYRMETGLULEUGLYTHRASNARG
16   ALAASPALAVALLEUHISASPTHRPROASN
17   ILELEUTYRPHEILELYSTHRALAGLYASN
18   GLYGLNPHELYSALAVALGLYASPSERLEU
19   GLUALAGLNGLNTYRGLYILEALAPHEPRO
20   LYSGLYSERASPGLULEUARGASPLYSVAL
21   ASNGLYALALEULYSTHRLEUARGGLUASN
22   GLYTHRTYRASNGLUILETYRLYSLYSTRP
23   PHEGLYTHRGLUPROLYS

Entity 2, L-glutamine - C5 H10 N2 O3 - 146.144 Da.

1   GLN

Samples:

sample_1: GlnBP, [U-100% 15N], 2 mM; potassium phosphate 20 mM; EDTA 2 mM; L-Glutamine 2.58 mM

sample_2: GlnBP, [U-100% 15N], 1 mM; potassium phosphate 20 mM; EDTA 2 mM; CHAPS 5 mM

sample_conditions_1: temperature: 288 K; pH: 7.2; pressure: 1 atm

sample_conditions_2: temperature: 291 K; pH: 7.2; pressure: 1 atm

sample_conditions_3: temperature: 294 K; pH: 7.2; pressure: 1 atm

sample_conditions_4: temperature: 303 K; pH: 7.2; pressure: 1 atm

sample_conditions_5: temperature: 306 K; pH: 7.2; pressure: 1 atm

sample_conditions_6: temperature: 310 K; pH: 7.2; pressure: 1 atm

sample_conditions_7: temperature: 313 K; pH: 7.2; pressure: 1 atm

sample_conditions_8: temperature: 298 K; pH: 7.2; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQC 1sample_1isotropicsample_conditions_1
1H-15N HSQC 2sample_1isotropicsample_conditions_2
1H-15N HSQC 3sample_1isotropicsample_conditions_3
1H-15N HSQC 4sample_1isotropicsample_conditions_4
1H-15N HSQC 5sample_1isotropicsample_conditions_5
1H-15N HSQC 6sample_1isotropicsample_conditions_6
1H-15N HSQC 7sample_1isotropicsample_conditions_7
1H-15N HSQC 8sample_2isotropicsample_conditions_1
1H-15N HSQC 9sample_2isotropicsample_conditions_2
1H-15N HSQC 10sample_2isotropicsample_conditions_3
1H-15N HSQC 11sample_2isotropicsample_conditions_8
1H-15N HSQC 12sample_2isotropicsample_conditions_4
1H-15N HSQC 13sample_2isotropicsample_conditions_5
1H-15N HSQC 14sample_2isotropicsample_conditions_6
1H-15N HSQC 15sample_2isotropicsample_conditions_7

Software:

SEDPHAT, Houtman, J. C. D., Brown, P. H., Bowden, B., Yamaguchi, H., Appella, E., Samelson, L. E., and Schuck, P. - Data fitting

NMR spectrometers:

  • NA NA NA MHz

Related Database Links:

UniProt BLAST P0AEQ3[23-248]
PDB
DBJ BAA35483 BAB34312 BAG76391 BAI24254 BAI29698
EMBL CAA32382 CAD05277 CAP75280 CAQ31312 CAQ88488
GB AAB49427 AAC73898 AAG55183 AAL19766 AAN42396
PIR AC0601
REF NP_308916 NP_415332 NP_455365 NP_459807 NP_706689
SP P0AEQ3 P0AEQ4 P0AEQ5