BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26512

Title: Thermal stability of chicken brain a-spectrin repeat 17: a spectroscopic study   PubMed: 22569754

Authors: Brenner, Annette; Kieffer, Bruno; Trave, Gilles; Froystein, Nils; Raae, Arnt

Citation: Brenner, Annette; Kieffer, Bruno; Trave, Gilles; Froystein, Nils; Raae, Arnt. "Thermal stability of chicken brain a-spectrin repeat 17: a spectroscopic study"  J. Biomol. NMR 53, 71-83 (2012).

Assembly members:
a-spectrin, polymer, 118 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
a-spectrin: MARGQRLEESLEYQQFVANV EEEEAWINEKMTLVASEDYG DTLAAIQGLLKKHEAFETDF TVHKDRVNDVCANGEDLIKK NNHHVENITAKMKGLKGKVS DLEKAAAQRKAKLDENSA

Data sets:
Data typeCount
13C chemical shifts25
T1 relaxation values65
T2 relaxation values65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1a-spectrin1

Entities:

Entity 1, a-spectrin 118 residues - Formula weight is not available

1   METALAARGGLYGLNARGLEUGLUGLUSER
2   LEUGLUTYRGLNGLNPHEVALALAASNVAL
3   GLUGLUGLUGLUALATRPILEASNGLULYS
4   METTHRLEUVALALASERGLUASPTYRGLY
5   ASPTHRLEUALAALAILEGLNGLYLEULEU
6   LYSLYSHISGLUALAPHEGLUTHRASPPHE
7   THRVALHISLYSASPARGVALASNASPVAL
8   CYSALAASNGLYGLUASPLEUILELYSLYS
9   ASNASNHISHISVALGLUASNILETHRALA
10   LYSMETLYSGLYLEULYSGLYLYSVALSER
11   ASPLEUGLULYSALAALAALAGLNARGLYS
12   ALALYSLEUASPGLUASNSERALA

Samples:

sample_1: a-spectrin, [U-100% 13C; U-100% 15N], 200 uM; D2O 10%; H2O 90%; Sodium phosphate buffer 20 mM; NaCl 0.1 M; DTT 1 mM; NaN3 0.05%

sample_conditions_1: temperature: 298 K; pH: 6.8; pressure: 1 atm

sample_conditions_2: temperature: 317 K; pH: 6.8; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - processing

CARA, Keller and Wuthrich - resonance assignment

CCPNMR, CCPN - data analysis

NMR spectrometers:

  • Bruker Biospin AV600 600 MHz

Related Database Links:

BMRB 18260