BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26032

Title: Structural insights of r(CGG) motif found in Fragile X Syndrome and Fragile-X associated tremor/ataxia syndrome (FXTAS) at 45 degree C

Authors: Kumar, Amit; Mishra, Subodh

Citation: Mishra, Subodh; Kumar, Amit. "Structural insights reveal the dynamics of the repeating r(CGG) motif found in Fragile X Syndrome and Fragile-X associated tremor/ataxia syndrome (FXTAS)"  Not known ., .-..

Assembly members:
RNA_(5'-R(P*CP*CP*GP*CP*GP*GP*CP*GP*G)-3'), polymer, 9 residues, 2901.816 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
RNA_(5'-R(P*CP*CP*GP*CP*GP*GP*CP*GP*G)-3'): CCGCGGCGG

Data sets:
Data typeCount
1H chemical shifts38

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (5'-R(P*CP*CP*GP*CP*GP*GP*CP*GP*G)-3')_11
2RNA (5'-R(P*CP*CP*GP*CP*GP*GP*CP*GP*G)-3')_21

Entities:

Entity 1, RNA (5'-R(P*CP*CP*GP*CP*GP*GP*CP*GP*G)-3')_1 9 residues - 2901.816 Da.

1   CCGCGGCGG

Samples:

CGG_45C: RNA (5'-R(P*CP*CP*GP*CP*GP*GP*CP*GP*G)-3') 1.5 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYCGG_45Cisotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - chemical shift assignment

SPARKY, Goddard - chemical shift assignment, peak picking

DISCOVER, Accelrys Software Inc. - geometry optimization

NMR spectrometers:

  • Bruker Avance 400 MHz

Related Database Links: