BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25996

Title: Solution structure of the J-K region of EMCV IRES   PubMed: 27525590

Authors: Imai, Shunsuke; D'Souza, Victoria; Wagner, Gerhard

Citation: Imai, Shunsuke; Hellen, Christopher; D'Souza, Victoria; Wagner, Gerhard. "An accurately preorganized IRES RNA structure enables eIF4G capture for initiation of viral translation"  Nat. Struct. Mol. Biol. 23, 859-864 (2016).

Assembly members:
RNA_(108-MER), polymer, 108 residues, 34838.840 Da.

Natural source:   Common Name: Encephalomyocarditis virus   Taxonomy ID: 12104   Superkingdom: Viruses   Kingdom: not available   Genus/species: Cardiovirus Cardiovirus A

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
RNA_(108-MER): GGGGCUGAAGGAUGCCCAGA AGGUACCCCAUUGUAUGGGA UCUGAUCUGGGGCCUCGGUG CACAUGCUUUACAUGUGUUU AGUCGAGGUUAAAAAACGUC UAGGCCCC

Data sets:
Data typeCount
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (108-MER)1

Entities:

Entity 1, RNA (108-MER) 108 residues - 34838.840 Da.

1   GGGGCUGAAG
2   GAUGCCCAGA
3   AGGUACCCCA
4   UUGUAUGGGA
5   UCUGAUCUGG
6   GGCCUCGGUG
7   CACAUGCUUU
8   ACAUGUGUUU
9   AGUCGAGGUU
10   AAAAAACGUC
11   UAGGCCCC

Samples:

sample_1: RNA (108-MER) 0.5 mM; potassium phosphate 10 mM; sodium chloride 10 mM; D2O, [U-2H], 100%

sample_2: RNA (108-MER), [U-2H, {H1',H2',H2,H8}-Ade], 0.5 mM; potassium phosphate 10 mM; sodium chloride 10 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

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