BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25968

Title: NMR resonance assignments of the apple allergen Mal d 1.0101   PubMed: 27165578

Authors: Ahammer, Linda; Tollinger, Martin; Grutsch, Sarina

Citation: Ahammer, Linda; Grutsch, Sarina; Tollinger, Martin. "NMR resonance assignments of the major apple allergen Mal d 1"  Biomol. NMR Assignments 10, 287-290 (2016).

Assembly members:
Mal_d_1.0101, polymer, 158 residues, Formula weight is not available

Natural source:   Common Name: apple   Taxonomy ID: 3750   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Malus domestica

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Mal_d_1.0101: GVYTFENEFTSEIPPSRLFK AFVLDADNLIPKIAPQAIKQ AEILEGNGGPGTIKKITFGE GSQYGYVKHRIDSIDEASYS YSYTLIEGDALTDTIEKISY ETKLVACGSGSTIKSISHYH TKGNIEIKEEHVKVGKEKAH GLFKLIESYLKDHPDAYN

Data sets:
Data typeCount
13C chemical shifts614
15N chemical shifts152
1H chemical shifts914

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mal d 1.01011

Entities:

Entity 1, Mal d 1.0101 158 residues - Formula weight is not available

1   GLYVALTYRTHRPHEGLUASNGLUPHETHR
2   SERGLUILEPROPROSERARGLEUPHELYS
3   ALAPHEVALLEUASPALAASPASNLEUILE
4   PROLYSILEALAPROGLNALAILELYSGLN
5   ALAGLUILELEUGLUGLYASNGLYGLYPRO
6   GLYTHRILELYSLYSILETHRPHEGLYGLU
7   GLYSERGLNTYRGLYTYRVALLYSHISARG
8   ILEASPSERILEASPGLUALASERTYRSER
9   TYRSERTYRTHRLEUILEGLUGLYASPALA
10   LEUTHRASPTHRILEGLULYSILESERTYR
11   GLUTHRLYSLEUVALALACYSGLYSERGLY
12   SERTHRILELYSSERILESERHISTYRHIS
13   THRLYSGLYASNILEGLUILELYSGLUGLU
14   HISVALLYSVALGLYLYSGLULYSALAHIS
15   GLYLEUPHELYSLEUILEGLUSERTYRLEU
16   LYSASPHISPROASPALATYRASN

Samples:

sample_1: Mal d 1.0101, [U-99% 13C; U-99% 15N], 0.65 mM; L-ascorbic acid 9.1 mM; sodium phosphate 10 mM

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCCO)NH-TOCSYsample_1isotropicsample_conditions_1
3D C,C-methylNOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian DD2 500 MHz
  • Bruker Avance II+ 600 MHz

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