BMRB Entry 25945

Title:
The NMR Structure of the Cdc42-interacting region of TOCA1
Deposition date:
2016-01-08
Original release date:
2016-05-23
Authors:
Mott, Helen; Watson, Joanna; Nietlispach, Daniel; Owen, Darerca
Citation:

Citation: Watson, Joanna; Nietlispach, Daniel; Owen, Darerca; Mott, Helen. "(1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1"  Biomol NMR Assign. 10, 407-411 (2016).
PubMed: 26988723

Assembly members:

Assembly members:
Toca1, polymer, 104 residues, 12092.6373 Da.

Natural source:

Natural source:   Common Name: Western clawed frog   Taxonomy ID: 8364   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus Xenopus (Silurana) tropicalis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Data typeCount
13C chemical shifts458
15N chemical shifts109
1H chemical shifts755

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Toca11

Entities:

Entity 1, Toca1 104 residues - 12092.6373 Da.

1   GLYPROLEUGLYSERHISMETLYSGLYPRO
2   ALALEUGLUASPPHESERHISLEUPROPRO
3   GLUGLNARGARGLYSARGLEUGLNGLNARG
4   ILEASPGLULEUSERARGGLULEUGLNLYS
5   GLUMETASPGLNLYSASPALALEUASNLYS
6   METLYSASPVALTYRGLULYSASNPROGLN
7   METGLYASPPROSERSERLEUHISPROLYS
8   ILEALAGLUTHRTHRSERASNILEGLUARG
9   LEUARGMETGLUILEHISLYSASNGLUALA
10   TRPLEUSERGLUVALGLUGLYLYSVALSER
11   GLNARGSERGLU

Samples:

HR1: Toca1, [U-13C; U-15N], 900.0 mM

sample_new_1: Toca1, [U-13C; U-15N], 900 mM

HR1_1: ionic strength: 0.150 M; pH: 7.500; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCHR1isotropicHR1_1
3D HNCAHR1isotropicHR1_1
3D HN(CO)CAHR1isotropicHR1_1
3D HNCACBHR1isotropicHR1_1
HN(CO)CACB (H[N[co[{CA|ca[C]}]]])HR1isotropicHR1_1
3D HNCOHR1isotropicHR1_1
3D HCCH-TOCSYHR1isotropicHR1_1
2D 1H-13C HSQC/HMQCHR1isotropicHR1_1
3D 1H-13C NOESYHR1isotropicHR1_1
3D 1H-15N NOESYHR1isotropicHR1_1
15NHSQCsample_new_1solutionHR1_1
HN(CO)CAsample_new_1solutionHR1_1

Software:

ARIA2.3 vany, Linge, O'Donoghue and Nilges - chemical shift assignment

AutoDep v4.3, PDBe - collection

CcpNmr_Analysis v2.2, CCPN - Spectrum analysis

aria v2.3, Michael Nilges - structure calculations

azara vany, Wayne Boucher - Spectrum analysis, Spectrum display

ccpNmr vany, CCPN - Spectrum analysis, Spectrum display

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 600 MHz
  • Bruker DRX 500 MHz
  • Bruker DRX 500 MHz

Related Database Links:

UniProt FBP1L_XENTR
AlphaFold Q28GK4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks