BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25849

Title: NMR Structure of the homeodomain transcription factor Gbx1[E17R,R52E} from Homo sapiens   PubMed: 27396829

Authors: Proudfoot, Andrew; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt

Citation: Proudfoot, Andrew; Geralt, Michael; Elsliger, Marc-Andre; Wilson, Ian; Wuthrich, Kurt; Serrano, Pedro. "Dynamic Local Polymorphisms in the Gbx1 Homeodomain Induced by DNA Binding"  Structure 24, 1372-1379 (2016).

Assembly members:
entity, polymer, 71 residues, 8316.757 Da.

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GAPGGKSRRRRTAFTSEQLL ELRKEFHCKKYLSLTERSQI AHALKLSEVQVKIWFQNERA KWKRIKAGNVS

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts76
1H chemical shifts462

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 71 residues - 8316.757 Da.

1   GLYALAPROGLYGLYLYSSERARGARGARG
2   ARGTHRALAPHETHRSERGLUGLNLEULEU
3   GLULEUARGLYSGLUPHEHISCYSLYSLYS
4   TYRLEUSERLEUTHRGLUARGSERGLNILE
5   ALAHISALALEULYSLEUSERGLUVALGLN
6   VALLYSILETRPPHEGLNASNGLUARGALA
7   LYSTRPLYSARGILELYSALAGLYASNVAL
8   SER

Samples:

sample_1: entity, [U-99% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.0798 M; pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

DYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

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