BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25785

Title: NMR Assignment and structure of CssA5 (middle region) of CssA thermometer from Neisseria meningitidis   PubMed: 27369378

Authors: Barnwal, Ravi; Godin, Kate; Varani, Gabriele

Citation: Barnwal, Ravi; Loh, Edmund; Godin, Kate; Yip, Jordan; Lavender, Hayley; Tang, Christoph; Varani, Gabriele. "Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion by Neisseria meningitidis"  Nucleic Acids Res. 44, 9426-9437 (2016).

Assembly members:
CssA5_RNA_(43-MER), polymer, 43 residues, 13741.223 Da.

Natural source:   Common Name: b-proteobacteria   Taxonomy ID: 487   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:   Production method: in vitro transcription

Entity Sequences (FASTA):
CssA5_RNA_(43-MER): GGUGAGUACGUAGAGUAUAC UUCGGUAUACUUAUAUACUU ACC

Data sets:
Data typeCount
13C chemical shifts32
15N chemical shifts18
1H chemical shifts204

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (43-MER)1

Entities:

Entity 1, RNA (43-MER) 43 residues - 13741.223 Da.

1   GGUGAGUACG
2   UAGAGUAUAC
3   UUCGGUAUAC
4   UUAUAUACUU
5   ACC

Samples:

sample_1: CssA5 RNA (43-MER)0.4 – 1.1 mM; CssA5 RNA (43-MER), 13C/15N-uniformly labeled, 0.4 – 1.1 mM; CssA5 RNA (43-MER), 13C/15N-AU labeled, 0.4 – 1.1 mM; CssA5 RNA (43-MER), partially deuterated, 0.4 – 1.1 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

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