BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25781

Title: NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer   PubMed: 27369378

Authors: Barnwal, Ravi; Godin, Kate; Varani, Gabriele; Loh, Edmund; Yip, Jordan; Tang, Christoph

Citation: Barnwal, Ravi; Loh, Edmund; Godin, Kate; Yip, Jordan; Lavender, Hayley; Tang, Christoph; Varani, Gabriele. "Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion by Neisseria meningitidis"  Nucleic Acids Res. 44, 9426-9437 (2016).

Assembly members:
CssA3_RNA_(42-MER), polymer, 42 residues, 13348.949 Da.

Natural source:   Common Name: b-proteobacteria   Taxonomy ID: 487   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:   Production method: in vitro transcription

Entity Sequences (FASTA):
CssA3_RNA_(42-MER): GGGUAGAGUAUAAUUAGUCU UCGGACUUCCUUAUACUUAU CC

Data sets:
Data typeCount
13C chemical shifts67
15N chemical shifts14
1H chemical shifts231

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (42-MER)1

Entities:

Entity 1, RNA (42-MER) 42 residues - 13348.949 Da.

1   GGGUAGAGUA
2   UAAUUAGUCU
3   UCGGACUUCC
4   UUAUACUUAU
5   CC

Samples:

sample_1: CssA3 RNA (42-MER)0.4 – 1.1 mM; CssA3 RNA (42-MER), 13C/15N-uniformly labeled, 0.4 – 1.1 mM; CssA3 RNA (42-MER), 13C/15N-AU labeled, 0.4 – 1.1 mM; CssA3 RNA (42-MER), partially deuterated, 0.4 – 1.1 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 280 K

sample_conditions_2: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
ARTSYsample_1isotropicsample_conditions_1
ARTSYsample_1anisotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz
  • Agilent INOVA 900 MHz

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