BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25777

Title: Solution NMR structure of Tetrahymena telomerase RNA pseudoknot   PubMed: 26472759

Authors: Cash, Darian; Feigon, Juli

Citation: Jiang, Jiansen; Chan, Henry; Cash, Darian; Miracco, Edward; Ogorzalek Loo, Rachel; Cascio, Dulio; O'Brien Johnson, Reid; Upton, Heather; Collins, Kathleen; Loo, Joseph; Zhou, Hong; Feigon, Juli. "Structure of Tetrahymena telomerase reveals new subunits, functions, and interactions"  Science 350, 4070-4070 (2015).

Assembly members:
RNA_(31-MER), polymer, 31 residues, 9888.990 Da.

Natural source:   Common Name: ciliates   Taxonomy ID: 5911   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Tetrahymena thermophila

Experimental source:   Production method: chemical synthesis   Host organism: in vitro transcription

Entity Sequences (FASTA):
RNA_(31-MER): XACCUUCACCAAUUAGGUUC AAAUAAGUGGU

Data sets:
Data typeCount
13C chemical shifts80
1H chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (31-MER)1

Entities:

Entity 1, RNA (31-MER) 31 residues - 9888.990 Da.

1   ADNACCUUCACC
2   AAUUAGGUUC
3   AAAUAAGUGG
4   U

Samples:

sample_1: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; H2O 90%; D2O 10%

sample_2_D2O: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; D2O 100%

sample_3_AU: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; ADENOSINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; URIDINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; H2O 90%; D2O 10%

sample_4_GC: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; CYTIDINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; GUANOSINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; H2O 90%; D2O 10%

sample_5_AUGC: RNA (31-MER) 10 mM; sodium phosphate 10 mM; KCl 50 mM; CYTIDINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; GUANOSINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; ADENOSINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; CYTIDINE-5'-MONOPHOSPHATE, [U-13C; U-15N], 100%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D imino NOESYsample_1isotropicsample_conditions_1
2D D2O NOESYsample_2_D2Oisotropicsample_conditions_1
2D 1H-13C HSQCsample_3_AUisotropicsample_conditions_1
2D 1H-13C HSQCsample_4_GCisotropicsample_conditions_1
2D TOCSYsample_2_D2Oisotropicsample_conditions_1
2D 1H-15N HSQCsample_3_AUisotropicsample_conditions_1
2D 1H-15N HSQCsample_4_GCisotropicsample_conditions_1
jNN-COSYsample_3_AUisotropicsample_conditions_1
jNN-COSYsample_4_GCisotropicsample_conditions_1
2D HCCH-COSYsample_3_AUisotropicsample_conditions_1
2D HCCH-COSYsample_4_GCisotropicsample_conditions_1
3D HCCH-TOCSYsample_3_AUisotropicsample_conditions_1
3D HCCH-TOCSYsample_4_GCisotropicsample_conditions_1
2D filtered/edited NOESYsample_3_AUisotropicsample_conditions_1
2D filtered/edited NOESYsample_4_GCisotropicsample_conditions_1
2D-HSQC-TROSYsample_5_AUGCisotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links: