BMRB Entry 25761

Title:
NMR structure of a DUF1491 family protein (CC_1065) from Caulobacter crescentus CB15
Deposition date:
2015-08-19
Original release date:
2015-10-12
Authors:
Qin, Haina; Serrano, Pedro; Wuthrich, Kurt
Citation:

Citation: Qin, Haina; Wuthrich, Kurt; Serrano, Pedro. "NMR structure of a DUF1491 family protein (CC_1065) from Caulobacter crescentus CB15"  to be published ., .-..

Assembly members:

Assembly members:
entity, polymer, 110 residues, 12262.887 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 190650   Superkingdom: Bacteria   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSpeedET

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts107
1H chemical shifts715

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 110 residues - 12262.887 Da.

1   GLYMETLEULEUSERTHRASPILETRPVAL
2   ALAALALEUILEARGARGALAGLULEUGLY
3   GLYALAPHEALATHRVALALAARGLYSGLY
4   ASPALAARGALAGLYALAVALLEUVALLYS
5   ALAVALASPARGARGGLUGLYTHRALAARG
6   LEUPHESERGLUALATHRARGGLYASPGLY
7   GLUARGPHETRPMETGLNPROVALARGSER
8   THRPHEGLUPROASPLEUASPALATYRALA
9   GLUARGALAALAARGILEASPPROASPILE
10   TRPVALVALGLUILEGLUASPARGASPGLY
11   ARGHISPHELEUTHRGLUPROVALGLUSER

Samples:

sample_1: entity, [U-98% 13C; U-98% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.240 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
APSY 4D-HACANHsample_1isotropicsample_conditions_1
APSY 5D-CBCACONHsample_1isotropicsample_conditions_1
APSY 5D-HACACONHsample_1isotropicsample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAK23049 ACL94583
REF NP_419881 WP_010918949 YP_002516491

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks