BMRB Entry 25617

Title:
Backbone 1H, 13C, and 15N chemical shift assignments for dimeric KaiB in complex with the CI domain of KaiC from the Thermosynechococcus elongatus BP-1 cyanobacterial species
Deposition date:
2015-05-15
Original release date:
2015-07-14
Authors:
Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy
Citation:

Citation: Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy. "A Protein Fold Switch Joins the Circadian Oscillator to Clock Output in Cyanobacteria"  Science 349, 324-328 (2015).
PubMed: 26113641

Assembly members:

Assembly members:
FTeKaiBN94YY, polymer, 102 residues, Formula weight is not available
FTeCI17247R41AK173AF, polymer, 247 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cyanobacteria   Taxonomy ID: 146786   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28b

Data sets:
Data typeCount
13C chemical shifts144
15N chemical shifts44
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dimeric KaiB, chain 11
2dimeric KaiB, chain 21
3CI2

Entities:

Entity 1, dimeric KaiB, chain 1 102 residues - Formula weight is not available

1   ASPTYRLYSASPASPASPASPLYSMETALA
2   PROLEUARGLYSTHRALAVALLEULYSLEU
3   TYRVALALAGLYASNTHRPROASNSERVAL
4   ARGALALEULYSTHRLEUASNASNILELEU
5   GLULYSGLUPHELYSGLYVALTYRALALEU
6   LYSVALILEASPVALLEULYSASNPROGLN
7   LEUALAGLUGLUASPLYSILELEUALATHR
8   PROTHRLEUALALYSVALLEUPROPROPRO
9   VALARGARGILEILEGLYASPLEUSERASN
10   ARGGLULYSVALLEUILEGLYLEUASPLEU
11   LEUALA

Entity 2, CI 247 residues - Formula weight is not available

1   ASPTYRLYSASPASPASPASPLYSALAGLU
2   VALLYSLYSILEPROTHRMETILEGLUGLY
3   PHEASPASPILESERHISGLYGLYLEUPRO
4   GLNGLYALATHRTHRLEUVALSERGLYTHR
5   SERGLYTHRGLYLYSTHRLEUPHEALAVAL
6   GLNPHELEUTYRASNGLYILETHRILEPHE
7   ASNGLUPROGLYILEPHEVALTHRPHEGLU
8   GLUSERPROGLNASPILEILELYSASNALA
9   LEUSERPHEGLYTRPASNLEUGLNSERLEU
10   ILEASPGLNGLYLYSLEUPHEILELEUASP
11   ALASERPROASPPROASPGLYGLNGLUVAL
12   ALAGLYASPPHEASPLEUSERALALEUILE
13   GLUARGILEGLNTYRALAILEARGLYSTYR
14   LYSALATHRARGVALSERILEASPSERVAL
15   THRALAVALPHEGLNGLNTYRASPALAALA
16   SERVALVALARGARGGLUILEPHEARGLEU
17   ALAPHEARGLEUALAGLNLEUGLYVALTHR
18   THRILEMETTHRTHRGLUARGVALASPGLU
19   TYRGLYPROVALALAARGPHEGLYVALGLU
20   GLUPHEVALSERASPASNVALVALILELEU
21   ARGASNVALLEUGLUGLYGLUARGARGARG
22   ARGTHRVALGLUILELEULYSLEUARGGLY
23   THRTHRHISMETLYSGLYGLUTYRPROPHE
24   THRILEASNASNGLYILEASNILEPHEASP
25   TYRLYSASPASPASPASPLYS

Samples:

sample_1: KaiB dimer, [U-13C; U-15N; U-2H], 550 uM; CI 560 uM; Protease Inhibitor Cocktail 550 uM; Tris 20 mM; MgCl2 1.5 mM; ADP 1.5 mM; NaCl 75 mM; DSS 10 uM; NaNa3 0.02%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.075 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D TROSY HNCACBsample_1isotropicsample_conditions_1
3D TROSY HN(CO)CACBsample_1isotropicsample_conditions_1
3D TROSY HNCAsample_1isotropicsample_conditions_1
3D TROSY HN(CO)CAsample_1isotropicsample_conditions_1
3D TROSY HN(CA)COsample_1isotropicsample_conditions_1
3D TROSY HNCOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

Mars, Young-Sang Jung and Markus Zweckstetter - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks