BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25549

Title: 1H, 13C, 15N backbone chemical shift assignments of mouse BMAL2 transactivation domain   PubMed: 25961797

Authors: Lee, Hsiau-Wei; Gustafson, Chelsea; Parsley, Nicole; Partch, Carrie

Citation: Xu, Haiyan; Guftafson, Chelsea; Sammons, Patrick; Khan, Sanjoy; Parsley, Nicole; Ramanathan, Chidambaram; Lee, Hsiau-Wei; Liu, Andrew; Partch, Carrie. "Cryptochrome 1 regulates the circadian clock through dynamic interactions with the BMAL1 C terminus"  Nat. Struct. Mol. Biol. 22, 476-484 (2015).

Assembly members:
mBMAL2-TAD, polymer, 46 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
mBMAL2-TAD: AMDPEFLGFDVLCDSDSIDM AAFMNYLEAEGGLGDPGDFS DIQWAL

Data sets:
Data typeCount
13C chemical shifts84
15N chemical shifts41
1H chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mBMAL2-TAD1

Entities:

Entity 1, mBMAL2-TAD 46 residues - Formula weight is not available

1   ALAMETASPPROGLUPHELEUGLYPHEASP
2   VALLEUCYSASPSERASPSERILEASPMET
3   ALAALAPHEMETASNTYRLEUGLUALAGLU
4   GLYGLYLEUGLYASPPROGLYASPPHESER
5   ASPILEGLNTRPALALEU

Samples:

sample_1: mBMAL2-TAD, [U-100% 13C; U-100% 15N], 0.3 mM; MES 10 mM; NaCl 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links: