BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25535

Title: Further exploration of the conformational space of alpha-synuclein fibrils: Solid-state NMR assignment of a high-pH polymorph   PubMed: 26318307

Authors: Verasdonck, Joeri; Bousset, Luc; Gath, Julia; Melki, Ronald; Boeckmann, Anja; Meier, Beat

Citation: Verasdonck, Joeri; Bousset, Luc; Gath, Julia; Melki, Ronald; Boeckmann, Anja; Meier, Beat. "Further exploration of the conformational space of alpha-synuclein fibrils: Solid-state NMR of a high-pH polymorph"  Biomol NMR Assign. 10, 5-12 (2016).

Assembly members:
alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
alpha-synuclein: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts207
15N chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-synuclein, 11
2alpha-synuclein, 21

Entities:

Entity 1, alpha-synuclein, 1 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: alpha-synuclein, [U-100% 13C; U-100% 15N], 25 mg

sample_conditions_1: pH: 9.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
DARRsample_1solidsample_conditions_1
NCAsample_1solidsample_conditions_1
NCOsample_1solidsample_conditions_1
NCACBsample_1solidsample_conditions_1
CANCOsample_1solidsample_conditions_1
NCOCAsample_1solidsample_conditions_1
NCACOsample_1solidsample_conditions_1
CCCsample_1solidsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 850 MHz

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