BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25466

Title: BCL-XL   PubMed: 25731750

Deposition date: 2015-02-04 Original release date: 2017-08-02

Authors: Yao, Yong; Marassi, Francesca

Citation: Yao, Yong; Fujimoto, Lynn; Hirshman, Nathan; Bobkov, Andrey; Antignani, Antonella; Youle, Richard; Marassi, Francesca. "Conformation of BCL-XL upon Membrane Integration."  J. Mol. Biol. 427, 2262-2270 (2015).

Assembly members:
BCL-XL, polymer, 205 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
BCL-XL: MAHHHHHHGSPEFMSQSNRE LVVDFLSYKLSQKGYSWSQF SDVEENRTEAPEGTESAVKQ ALREAGDEFELRYRRAFSDL TSQLHITPGTAYQSFEQVVN ELFRDGVNWGRIVAFFSFGG ALCVESVDKEMQVLVSRIAA WMATYLNDHLEPWIQENGGW DTFVELYGNNAAAESRKGQE RFNRWFLTGMTVAGVVLLGS LFSRK

Data sets:
Data typeCount
13C chemical shifts295
15N chemical shifts150
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1BCL-XL1

Entities:

Entity 1, BCL-XL 205 residues - Formula weight is not available

1   METALAHISHISHISHISHISHISGLYSER
2   PROGLUPHEMETSERGLNSERASNARGGLU
3   LEUVALVALASPPHELEUSERTYRLYSLEU
4   SERGLNLYSGLYTYRSERTRPSERGLNPHE
5   SERASPVALGLUGLUASNARGTHRGLUALA
6   PROGLUGLYTHRGLUSERALAVALLYSGLN
7   ALALEUARGGLUALAGLYASPGLUPHEGLU
8   LEUARGTYRARGARGALAPHESERASPLEU
9   THRSERGLNLEUHISILETHRPROGLYTHR
10   ALATYRGLNSERPHEGLUGLNVALVALASN
11   GLULEUPHEARGASPGLYVALASNTRPGLY
12   ARGILEVALALAPHEPHESERPHEGLYGLY
13   ALALEUCYSVALGLUSERVALASPLYSGLU
14   METGLNVALLEUVALSERARGILEALAALA
15   TRPMETALATHRTYRLEUASNASPHISLEU
16   GLUPROTRPILEGLNGLUASNGLYGLYTRP
17   ASPTHRPHEVALGLULEUTYRGLYASNASN
18   ALAALAALAGLUSERARGLYSGLYGLNGLU
19   ARGPHEASNARGTRPPHELEUTHRGLYMET
20   THRVALALAGLYVALVALLEULEUGLYSER
21   LEUPHESERARGLYS

Samples:

sample_1: BCL-XL, [U-99% 13C; U-99% 15N], 0.1 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 0.025 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts