BMRB Entry 25453

Name: 1H, 13C and 15N assignment of the C-terminal domain of human galectin 8
Published: 2015-08-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25453

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N assignment of the C-terminal domain of human galectin 8

Deposition date:

2015-01-26

Original release date:

2015-08-13

Authors:

Hsu, Shang-Te Danny; Liu, Chun-Hao Gerard; Chien, Chih-Ta Henry

Citation:

Citation: Liu, Chun-Hao Gerard; Chien, Chih-Ta Henry; Lin, Chun-Hung; Hsu, Shang-Te Danny. "NMR assignments of the C-terminal domain of human galectin-8" Biomol. NMR Assign. 9, 427-430 (2015).
PubMed: 26126590

Assembly members:

Assembly members:
G8C, polymer, 155 residues, 17590.0 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
G8C: MGSSHHHHHHSSGLVPRGSH MRLPFAARLNTPMGPGRTVV VKGEVNANAKSFNVDLLAGK SKDIALHLNPRLNIKAFVRN SFLQESWGEEERNITSFPFS PGMYFEMIIYCDVREFKVAV NGVHSLEYKHRFKELSSIDT LEINGDIHLLEVRSW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	537
15N chemical shifts	129
1H chemical shifts	789

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human galectin-8	1

Entities:

Entity 1, Human galectin-8 155 residues - 17590.0 Da.

Residues 1-20 represent a His-tag

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

ARG

LEU

PRO

PHE

ALA

ARG

LEU

ASN

4

THR

PRO

MET

GLY

PRO

GLY

ARG

THR

VAL

5

VAL

LYS

GLY

GLU

VAL

ASN

ALA

ASN

ALA

LYS

6

SER

PHE

ASN

VAL

ASP

LEU

ALA

GLY

LYS

7

SER

LYS

ASP

ILE

ALA

LEU

HIS

LEU

ASN

PRO

8

ARG

LEU

ASN

ILE

LYS

ALA

PHE

VAL

ARG

ASN

9

SER

PHE

LEU

GLN

GLU

SER

TRP

GLY

GLU

10

GLU

ARG

ASN

ILE

THR

SER

PHE

PRO

PHE

SER

11

PRO

GLY

MET

TYR

PHE

GLU

MET

ILE

TYR

12

CYS

ASP

VAL

ARG

GLU

PHE

LYS

VAL

ALA

VAL

13

ASN

GLY

VAL

HIS

SER

LEU

GLU

TYR

LYS

HIS

14

ARG

PHE

LYS

GLU

LEU

SER

ILE

ASP

THR

15

LEU

GLU

ILE

ASN

GLY

ASP

ILE

HIS

LEU

16

GLU

VAL

ARG

SER

TRP

Samples:

sample_1: G8C, [U-98% 13C; U-98% 15N], 100 ± 20 uM; H2O 90%; D2O 10%; phosphate buffer 0.01 M; potassium chloride 0.0027 M; sodium chloride 0.137 M; NaN3 0.02%

sample_conditions_1: ionic strength: 0.1715 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

Bruker Avance 850 MHz
Bruker Avance 600 MHz

PDB	2YRO
EMBL	X91790.1