BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25440

Title: 1H and 113Cd chemical shift assignments for the cysteins and metals of Bud31p Cd3   PubMed: 25703931

Authors: van Roon, Anne-Marie; Yang, Ji-Chun; Mathieu, Daniel; Bermel, Wolfgang; Nagai, Kiyoshi; Neuhaus, David

Citation: van Roon, Anne-Marie; Yang, Ji-Chun; Mathieu, Daniel; Bermel, Wolfgang; Nagai, Kiyoshi; Neuhaus, David. "113 Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p"  Angew. Chem. Int. Ed. Engl. 54, 4861-4864 (2015).

Assembly members:
Bud31p_polypeptide, polymer, 159 residues, 18554.609 Da.
CADMIUM ION, non-polymer, 112.411 Da.

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Bud31p_polypeptide: GGSPRIKTRRSKPAPDGFEK IKPTLTDFEIQLRDAQKDKS SKLAAKSNEQLWEIMQLHHQ RSRYIYTLYYKRKAISKDLY DWLIKEKYADKLLIAKWRKT GYEKLCCLRCIQKNETNNGS TCICRVPRAQLEEEARKKGT QVSFHQCVHCGCRGCASTD

Data sets:
Data typeCount
113Cd chemical shifts6
1H chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bud31p polypeptide1
2Cadmium 12
3Cadmium 22
4Cadmium 32

Entities:

Entity 1, Bud31p polypeptide 159 residues - 18554.609 Da.

1   GLYGLYSERPROARGILELYSTHRARGARG
2   SERLYSPROALAPROASPGLYPHEGLULYS
3   ILELYSPROTHRLEUTHRASPPHEGLUILE
4   GLNLEUARGASPALAGLNLYSASPLYSSER
5   SERLYSLEUALAALALYSSERASNGLUGLN
6   LEUTRPGLUILEMETGLNLEUHISHISGLN
7   ARGSERARGTYRILETYRTHRLEUTYRTYR
8   LYSARGLYSALAILESERLYSASPLEUTYR
9   ASPTRPLEUILELYSGLULYSTYRALAASP
10   LYSLEULEUILEALALYSTRPARGLYSTHR
11   GLYTYRGLULYSLEUCYSCYSLEUARGCYS
12   ILEGLNLYSASNGLUTHRASNASNGLYSER
13   THRCYSILECYSARGVALPROARGALAGLN
14   LEUGLUGLUGLUALAARGLYSLYSGLYTHR
15   GLNVALSERPHEHISGLNCYSVALHISCYS
16   GLYCYSARGGLYCYSALASERTHRASP

Entity 2, Cadmium 1 - Cd - 112.411 Da.

1   CD

Samples:

sample_1: Bud31p (113Cd)3 2.6 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT, [U-2H], 5 mM; D2O 100%

sample_conditions_1: ionic strength: 0.27 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.27 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 113Cd-113Cd COSYsample_1isotropicsample_conditions_1
2D 113Cd-1H HSQC NOESYsample_1isotropicsample_conditions_1
2D 113Cd-1H HMQC-RELAYsample_1isotropicsample_conditions_2
2D 1H-13C HSQC aliphatic (natural abundance)sample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphatic (natural abundance)sample_1isotropicsample_conditions_2

Software:

TOPSPIN v3.1, Bruker Biospin - processing

SPARKY v3.115, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance AVIII 850 MHz
  • Bruker Avance AVIII 500 MHz

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