BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25436

Title: Solution structure of hnRNP C RRM in complex with the 5'-AUUUUUC-3' RNA   PubMed: 25216038

Authors: Cienikova, Zuzana; Damberger, Fred; Hall, Jonathan; Allain, Frederic; Maris, Christophe

Citation: Cienikova, Zuzana; Damberger, Fred; Hall, Jonathan; Allain, Frederic; Maris, Christophe. "Structural and mechanistic insights into poly(uridine) tract recognition by the hnRNP C RNA recognition motif."  J. Am. Chem. Soc. 136, 14536-14544 (2014).

Assembly members:
hnRNP_C, polymer, 105 residues, 11292.014 Da.
RNA_(5'-R(*AP*UP*UP*UP*UP*UP*C)-3'), polymer, 7 residues, 2120.276 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hnRNP_C: ASNVTNKTDPRSMNSRVFIG NLNTLVVKKSDVEAIFSKYG KIVGCSVHKGFAFVQYVNER NARAAVAGEDGRMIAGQVLD INLAAEPKVNRGKAGVKRSA AEMYG
RNA_(5'-R(*AP*UP*UP*UP*UP*UP*C)-3'): AUUUUUC

Data sets:
Data typeCount
13C chemical shifts464
15N chemical shifts117
1H chemical shifts790

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hnRNP_C1
2RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3')2

Entities:

Entity 1, hnRNP_C 105 residues - 11292.014 Da.

1   ALASERASNVALTHRASNLYSTHRASPPRO
2   ARGSERMETASNSERARGVALPHEILEGLY
3   ASNLEUASNTHRLEUVALVALLYSLYSSER
4   ASPVALGLUALAILEPHESERLYSTYRGLY
5   LYSILEVALGLYCYSSERVALHISLYSGLY
6   PHEALAPHEVALGLNTYRVALASNGLUARG
7   ASNALAARGALAALAVALALAGLYGLUASP
8   GLYARGMETILEALAGLYGLNVALLEUASP
9   ILEASNLEUALAALAGLUPROLYSVALASN
10   ARGGLYLYSALAGLYVALLYSARGSERALA
11   ALAGLUMETTYRGLY

Entity 2, RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') 7 residues - 2120.276 Da.

1   AUUUUUC

Samples:

sample_1: hnRNP C, [U-100% 15N], 1.0 mM; RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') 1.0 mM; sodium phosphate 20 mM; sodium chloride 10 mM; H2O 90%; D2O 10%

sample_2: hnRNP C, [U-100% 13C; U-100% 15N], 1.0 mM; RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') 1.0 mM; sodium phosphate 20 mM; sodium chloride 10 mM; H2O 90%; D2O 10%

sample_3: hnRNP C, [U-100% 13C; U-100% 15N], 1.0 mM; RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') 1.0 mM; sodium phosphate 20 mM; sodium chloride 10 mM; D2O 100%

sample_4: hnRNP C, [U-100% 15N], 0.5 – 1.0 mM; RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3'), [100% 13C5]-Ura, 0.5 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 10 mM; D2O 100%

sample_conditions_1: ionic strength: 0.040 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C edited-filtered NOESYsample_4isotropicsample_conditions_1
3D 1H-13C edited-filtered NOESYsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker AVIII 600 MHz
  • Bruker AVIII 700 MHz
  • Bruker AVIII 500 MHz
  • Bruker AVIII 750 MHz

Related Database Links:

UNP P07910
BMRB 25469
PDB
DBJ BAB27553 BAB28370 BAB31934 BAC40233 BAC40499
EMBL CAD61934 CAD62326 CAH91328
GB AAA36576 AAA52680 AAC61695 AAD03717 AAH03394
REF NP_001029567 NP_001070910 NP_001070911 NP_001075507 NP_001125784
SP G3V9R8 O77768 P07910 Q5RA82 Q9Z204
TPG DAA25480