BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25428

Title: NMR structure of the acidic domain of SYNCRIP (hnRNPQ)   PubMed: 27081926

Authors: Serrano, Pedro; Wuthrich, Kurt; Beuck, Christine

Citation: Beuck, Christine; Williamson, James; Wuthrich, Kurt; Serrano, Pedro. "The acidic domain is a unique structural feature of the splicing factor SYNCRIP"  Protein Sci. 25, 1545-1550 (2016).

Assembly members:
entity, polymer, 85 residues, 9494.729 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GSENFQTLLDAGLPQKVAEK LDEIYVAGLVAHSDLDERAI EALKEFNEDGALAVLQQFKD SDLSHVQNKSAFLCGVMKTY RQREK

Data sets:
Data typeCount
13C chemical shifts339
15N chemical shifts92
1H chemical shifts591

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 85 residues - 9494.729 Da.

1   GLYSERGLUASNPHEGLNTHRLEULEUASP
2   ALAGLYLEUPROGLNLYSVALALAGLULYS
3   LEUASPGLUILETYRVALALAGLYLEUVAL
4   ALAHISSERASPLEUASPGLUARGALAILE
5   GLUALALEULYSGLUPHEASNGLUASPGLY
6   ALALEUALAVALLEUGLNGLNPHELYSASP
7   SERASPLEUSERHISVALGLNASNLYSSER
8   ALAPHELEUCYSGLYVALMETLYSTHRTYR
9   ARGGLNARGGLULYS

Samples:

sample_1: entity, [U-99% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.0798 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
APSY 4D-HACANHsample_1isotropicsample_conditions_1
APSY 5D-HACACONHsample_1isotropicsample_conditions_1
APSY 5D-CBCACONHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

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