BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25424

Title: The solution structure of DEFA1, a highly potent antimicrobial peptide from the horse   PubMed: 25769951

Authors: Jung, Sascha; Michalek, Matthias; Shomali, Mohammad; Soennichsen, Frank

Citation: Jung, Sascha; Michalek, Matthias; Soennichsen, Frank; Shomali, Mohammad; Cauchard, Severine; Groetzinger, Joachim. "Solution structure and functional studies of the highly potent equine antimicrobial peptide DEFA1"  Biochem. Biophys. Res. Commun. 459, 668-672 (2015).

Assembly members:
entity, polymer, 34 residues, 4086.812 Da.

Natural source:   Common Name: Domestic horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus ferus

Experimental source:   Production method: chemical synthesis   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: SCTCRRAWICRWGERHSGKC IDQKGSTYRLCCRR

Data sets:
Data typeCount
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 34 residues - 4086.812 Da.

1   SERCYSTHRCYSARGARGALATRPILECYS
2   ARGTRPGLYGLUARGHISSERGLYLYSCYS
3   ILEASPGLNLYSGLYSERTHRTYRARGLEU
4   CYSCYSARGARG

Samples:

sample_1: entity 0.5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.02 M; pH: 5.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
GB ABP96800 ACV49728
REF NP_001159560