BMRB Entry 25416

Title:
Structural features of a 3' splice site in influenza A: 39-nt hairpin
Deposition date:
2015-01-06
Original release date:
2015-05-04
Authors:
Turner, Douglas; Kennedy, Scott; Chen, Jonathan
Citation:

Citation: Chen, Jonathan; Kennedy, Scott; Turner, Douglas. "Structural features of a 3' splice site in influenza A"  Biochemistry 54, 3269-3285 (2015).
PubMed: 25909229

Assembly members:

Assembly members:
RNA_(39-MER), polymer, 39 residues, 12688.705 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: pUC18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_(39-MER): GGAUUUGCAGGCCUACCAGA AACGGAUGGGAGUGCAGAU

Data sets:
Data typeCount
1H chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 39 residues - 12688.705 Da.

1   GGAUUUGCAG
2   GCCUACCAGA
3   AACGGAUGGG
4   AGUGCAGAU

Samples:

sample_1: entity 1.1 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 293.15 K

sample_conditions_2: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 271.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

AMBER v11, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz