BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25414

Title: Structural features of a 3' splice site influenza A: 11-nt hairpin   PubMed: 25909229

Authors: Turner, Douglas; Kennedy, Scott; Chen, Jonathan

Citation: Chen, Jonathan; Kennedy, Scott; Turner, Douglas. "Structural features of a 3' splice site in influenza A"  Biochemistry 54, 3269-3285 (2015).

Assembly members:
RNA_(11-MER), polymer, 11 residues, 3552.259 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
RNA_(11-MER): CCAGAAACGGA

Data sets:
Data typeCount
1H chemical shifts97
31P chemical shifts10

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 11 residues - 3552.259 Da.

1   CCAGAAACGG
2   A

Samples:

sample_1: entity 1.0 mM; H2O 95%; D2O 5%

sample_2: entity 4.0 mM; D2O 100%

sample_conditions_1: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 293.15 K

sample_conditions_2: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 275.15 K

sample_conditions_3: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 272.15 K

sample_conditions_4: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 271.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_3
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_4
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_4
2D 1H-31P HETCORsample_2isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

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