BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25354

Title: SH3 domain from yeast Abp1p   PubMed: 25569628

Authors: Lundstrom, Patrik

Citation: Niklasson, Markus; Ahlner, Alexandra; Andresen, Cecilia; Marsh, Joseph; Lundstrom, Patrik. "Fast and accurate resonance assignment of small-to-large proteins by combining automated and manual approaches"  PLOS Comput. Biol. 11, e1004022-e1004022 (2015).

Assembly members:
Abp1p_SH3, polymer, 62 residues, Formula weight is not available

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Abp1p_SH3: GAMAPWATAEYDYDAAEDNE LTFVENDKIINIEFVDDDWW LGELEKDGSKGLFPSNYVSL GN

Data sets:
Data typeCount
13C chemical shifts215
15N chemical shifts57
1H chemical shifts311

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abp1p SH3 domain1

Entities:

Entity 1, Abp1p SH3 domain 62 residues - Formula weight is not available

1   GLYALAMETALAPROTRPALATHRALAGLU
2   TYRASPTYRASPALAALAGLUASPASNGLU
3   LEUTHRPHEVALGLUASNASPLYSILEILE
4   ASNILEGLUPHEVALASPASPASPTRPTRP
5   LEUGLYGLULEUGLULYSASPGLYSERLYS
6   GLYLEUPHEPROSERASNTYRVALSERLEU
7   GLYASN

Samples:

sample_1: Abp1p SH3, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

COMPASS, Niklasson, Ahlner, Andresen, Marsh, Lundstrom - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16843 18054 18055 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 18078 26519
PDB
DBJ GAA21988
EMBL CAA36075 CAA42253 CAY78291
GB AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991
PRF 1603360A
REF NP_010012
SP P15891
TPG DAA07557