BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25247

Title: Chemical Shift 1H, 13C, 15N Assignments of FliG bound to unlabeled FliF C-terminal peptide   PubMed: 28089452

Deposition date: 2014-09-24 Original release date: 2019-07-11

Authors: Levenson, Robert

Citation: Lynch, Michael; Levenson, Robert; Kim, Eun; Sircar, Ria; Blair, David; Dahlquist, Frederick; Crane, Brian. "Co-Folding of a FliF-FliG Split Domain Forms the Basis of the MS:C Ring Interface within the Bacterial Flagellar Motor"  Structure 25, 317-328 (2017).

Assembly members:
FliFc, polymer, 38 residues, Formula weight is not available
FliG, polymer, 342 residues, Formula weight is not available

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
FliFc: VSPEEKELLELLEELENIFS RSPSDIAEIVRLWFFERG
FliG: MHHHQHHMPEKKIDGRRKAA VLLVALGPEKAAQVMKHLDE ETVEQLVVEIANIGRVTPEE KKQVLEEFLSLAKAKEMISE GGIEYAKKVLEKAFGPERAR KIIERLTSSLQVKPFSFVRD TDPVQLVNFLQSEHPQTIAV VLSYLDPPVAAQILGALPEE LQTEVLKRIALLERTSPEVV KEIERNLEKKISGFVSRTFS KVGGIDTAAEIMNNLDRTTE KKIMDKLVQENPELADEIRR RMFVFEDILKLDDRSIQLVL REVDTRDLALALKGASDELK EKIFKNMSKRAAALLKDELE YMGPVRLKDVEEAQQKIINI IRRLEEAGEIVIARGGGEEL IM

Data sets:
Data typeCount
13C chemical shifts557
15N chemical shifts273
1H chemical shifts273

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FliFc1
2FliG2

Entities:

Entity 1, FliFc 38 residues - Formula weight is not available

Corresponds to cytoplasmic C-terminal region of FliF

1   VALSERPROGLUGLULYSGLULEULEUGLU
2   LEULEUGLUGLULEUGLUASNILEPHESER
3   ARGSERPROSERASPILEALAGLUILEVAL
4   ARGLEUTRPPHEPHEGLUARGGLY

Entity 2, FliG 342 residues - Formula weight is not available

N-terminal His-tagged FliG construct.

1   METHISHISHISGLNHISHISMETPROGLU
2   LYSLYSILEASPGLYARGARGLYSALAALA
3   VALLEULEUVALALALEUGLYPROGLULYS
4   ALAALAGLNVALMETLYSHISLEUASPGLU
5   GLUTHRVALGLUGLNLEUVALVALGLUILE
6   ALAASNILEGLYARGVALTHRPROGLUGLU
7   LYSLYSGLNVALLEUGLUGLUPHELEUSER
8   LEUALALYSALALYSGLUMETILESERGLU
9   GLYGLYILEGLUTYRALALYSLYSVALLEU
10   GLULYSALAPHEGLYPROGLUARGALAARG
11   LYSILEILEGLUARGLEUTHRSERSERLEU
12   GLNVALLYSPROPHESERPHEVALARGASP
13   THRASPPROVALGLNLEUVALASNPHELEU
14   GLNSERGLUHISPROGLNTHRILEALAVAL
15   VALLEUSERTYRLEUASPPROPROVALALA
16   ALAGLNILELEUGLYALALEUPROGLUGLU
17   LEUGLNTHRGLUVALLEULYSARGILEALA
18   LEULEUGLUARGTHRSERPROGLUVALVAL
19   LYSGLUILEGLUARGASNLEUGLULYSLYS
20   ILESERGLYPHEVALSERARGTHRPHESER
21   LYSVALGLYGLYILEASPTHRALAALAGLU
22   ILEMETASNASNLEUASPARGTHRTHRGLU
23   LYSLYSILEMETASPLYSLEUVALGLNGLU
24   ASNPROGLULEUALAASPGLUILEARGARG
25   ARGMETPHEVALPHEGLUASPILELEULYS
26   LEUASPASPARGSERILEGLNLEUVALLEU
27   ARGGLUVALASPTHRARGASPLEUALALEU
28   ALALEULYSGLYALASERASPGLULEULYS
29   GLULYSILEPHELYSASNMETSERLYSARG
30   ALAALAALALEULEULYSASPGLULEUGLU
31   TYRMETGLYPROVALARGLEULYSASPVAL
32   GLUGLUALAGLNGLNLYSILEILEASNILE
33   ILEARGARGLEUGLUGLUALAGLYGLUILE
34   VALILEALAARGGLYGLYGLYGLUGLULEU
35   ILEMET

Samples:

FliG-FliFc: FliFc 350 ± 50 mM; FliG, [U-13C; U-15N; U-2H], 350 ± 50 mM; Sodium phosphate 50 mM; NaCl 100 mM; EDTA 1 mM

Sodium_Phosphate: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCFliG-FliFcisotropicSodium_Phosphate
3D HNCAFliG-FliFcisotropicSodium_Phosphate
3D HNCACBFliG-FliFcisotropicSodium_Phosphate
3D HN(CO)CAFliG-FliFcisotropicSodium_Phosphate
3D HN(COCA)CBFliG-FliFcisotropicSodium_Phosphate

Software:

ANSIG, Kraulis - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts