BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25148

Title: NTD-NUSA

Authors: Mobli, Mehdi; Lewis, Peter

Citation: Mobli, Mehdi; Ma, Cong; Yang, Xiao; Keller, Andrew; King, Glenn; Lewis, Peter. "The Mechanism Of Transcription Pausing Enhancement By NusA"  Not known ., .-..

Assembly members:
NTD-NUSA, polymer, 124 residues, 14019.991 Da.

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NTD-NUSA: MSSELLDALTILEKEKGISK EIIIEAIEAALISAYKRNFN QAQNVRVDLNRETGSIRVFA RKDVVDEVYDQRLEISIEEA QGIHPEYMVGDVVEIEVTPK DFGRIAAQTAKQVVTQRVRE AERG

Data sets:
Data typeCount
1H chemical shifts878
13C chemical shifts530
15N chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NTD-NUSA1

Entities:

Entity 1, NTD-NUSA 124 residues - 14019.991 Da.

1   METSERSERGLULEULEUASPALALEUTHR
2   ILELEUGLULYSGLULYSGLYILESERLYS
3   GLUILEILEILEGLUALAILEGLUALAALA
4   LEUILESERALATYRLYSARGASNPHEASN
5   GLNALAGLNASNVALARGVALASPLEUASN
6   ARGGLUTHRGLYSERILEARGVALPHEALA
7   ARGLYSASPVALVALASPGLUVALTYRASP
8   GLNARGLEUGLUILESERILEGLUGLUALA
9   GLNGLYILEHISPROGLUTYRMETVALGLY
10   ASPVALVALGLUILEGLUVALTHRPROLYS
11   ASPPHEGLYARGILEALAALAGLNTHRALA
12   LYSGLNVALVALTHRGLNARGVALARGGLU
13   ALAGLUARGGLY

Samples:

sample_1: NTD-NUSA, [U-99% 13C; U-99% 15N], 400 uM; HEPES 10 mM; sodium chloride 150 mM

sample_conditions_1: temperature: 298 K; pH: 7.8; pressure: 1 atm; ionic strength: 0.16 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN v3.1, Bruker Biospin - collection

Rowland_NMR_Toolkit v3, J. C. Hoch & A. S. Stern - processing

TALOS v+, Cornilescu, Delaglio and Bax - data analysis

CCPNMR v2.2, CCPN - peak picking

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAI85290 BAM52307 BAM57883 GAK80641
EMBL CAA79231 CAB13533 CCU58233 CEI56842 CEJ77249
GB ADM37756 ADV96687 AEP86650 AEP90813 AFI28344
REF NP_389542 WP_003220941 WP_003231912 WP_010328008 WP_010334282
SP P32727