BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25121

Title: The influenza hemagglutinin fusion domain is an amphipathic helical-hairpin that functions by inducing negative membrane curvature   PubMed: 25398882

Authors: Smrt, Sean; Draney, Adrian; Lorieau, Justin

Citation: Smrt, Sean; Draney, Adrian; Lorieau, Justin. "The influenza hemagglutinin fusion domain is an amphipathic helical hairpin that functions by inducing membrane curvature"  J. Biol. Chem. 290, 228-238 (2015).

Assembly members:
HAfp23-F3G, polymer, 30 residues, Formula weight is not available

Natural source:   Common Name: Influenza Virus Type A   Taxonomy ID: 11320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Influenzavirus A not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HAfp23-F3G: GLGGAIAGFIEGGWTGMIDG WYGGSKKKKD

Data sets:
Data typeCount
15N chemical shifts48
1H chemical shifts48
H exchange rates21
T1 relaxation values42
T2 relaxation values42
heteronuclear NOE values42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HAfp23-F3G1

Entities:

Entity 1, HAfp23-F3G 30 residues - Formula weight is not available

1   GLYLEUGLYGLYALAILEALAGLYPHEILE
2   GLUGLYGLYTRPTHRGLYMETILEASPGLY
3   TRPTYRGLYGLYSERLYSLYSLYSLYSASP

Samples:

pH_7.4_sample: HAfp23-F3G, [U-100% 15N], 850 ± 150 uM; TRIS 25 ± 1 mM; DPC 150 ± 10 mM; D2O 10%; H2O 90%

pH_4.2_sample: HAfp23-F3G, [U-100% 15N], 600 ± 150 uM; Citrate 25 ± 1 mM; DPC 150 ± 10 mM; D2O 10%; H2O 90%

pH_7.4_Sample_condition: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 305 K

pH_4.2_Sample_condition: ionic strength: 0 M; pH: 4.2; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCpH_7.4_sampleisotropicpH_7.4_Sample_condition
2D 1H-15N HSQCpH_4.2_sampleisotropicpH_4.2_Sample_condition

Software:

SPARKY, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMR spectrometers:

  • Bruker Avance 500 MHz

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