BMRB Entry 25120

Title:
Human cardiac troponin C (aCys) in complex with cTnI[1-73] and cTnI[144-163]
Deposition date:
2014-07-31
Original release date:
2014-09-03
Authors:
Hwang, Peter
Citation:

Citation: Hwang, Peter. "The intrinsically disordered cardiac-specific N-terminal region of troponin I positions the regulatory domain of troponin C"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
cTnC, polymer, 161 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3

Data sets:
Data typeCount
1H chemical shifts908
13C chemical shifts615
15N chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cTnC-cTnI[1-73]-cTnI[144-163]1

Entities:

Entity 1, cTnC-cTnI[1-73]-cTnI[144-163] 161 residues - Formula weight is not available

1   METASPASPILETYRLYSALAALAVALGLU
2   GLNLEUTHRGLUGLUGLNLYSASNGLUPHE
3   LYSALAALAPHEASPILEPHEVALLEUGLY
4   ALAGLUASPGLYSERILESERTHRLYSGLU
5   LEUGLYLYSVALMETARGMETLEUGLYGLN
6   ASNPROTHRPROGLUGLULEUGLNGLUMET
7   ILEASPGLUVALASPGLUASPGLYSERGLY
8   THRVALASPPHEASPGLUPHELEUVALMET
9   METVALARGSERMETLYSASPASPSERLYS
10   GLYLYSSERGLUGLUGLULEUSERASPLEU
11   PHEARGMETPHEASPLYSASNALAASPGLY
12   TYRILEASPLEUASPGLULEULYSILEMET
13   LEUGLNALATHRGLYGLUTHRILETHRGLU
14   ASPASPILEGLUGLULEUMETLYSASPGLY
15   ASPLYSASNASNASPGLYARGILEASPTYR
16   ASPGLUPHELEUGLUPHEMETLYSGLYVAL
17   GLU

Samples:

sample_1: cTnC, [U-100% 13C; U-100% 15N], 0.5 mM; cTnI[1-73] 0.5 mM; cTnI[144-163], [U-100% 15N], 1 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 313 K; pH: 6.2; pressure: 1 atm; ionic strength: 0.12 M

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 15385 15388 15400 15427 16190 16752 17103 19789 25034 25035 25495 25797
PDB
DBJ BAA02369 BAG36483
EMBL CAA30736 CAG46663 CAG46683
GB AAA36772 AAA37492 AAA37493 AAA48654 AAB91994
PIR TPHUCC
PRF 1510257A 750650A
REF NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_001291793
SP P02591 P09860 P19123 P63315 P63316
TPG DAA16908
AlphaFold P02591 P09860 P63316 P19123 P63315

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks