BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25076

Title: Solid-state NMR resonance assignments of the filament-forming CARD domain of the innate immunity signaling protein MAVS

Authors: He, Lichun; Luehrs, Thorsten; Ritter, Christiane

Citation: He, Lichun; Luehrs, Thorsten; Ritter, Christiane. "Solid-state NMR resonance assignments of the filament-forming CARD domain of the innate immunity signaling protein MAVS"  Biomol. NMR Assignments ., .-..

Assembly members:
MAVS_CARD, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MAVS_CARD: GSMPFAEDKTYKYICRNFSN FCNVDVVEILPYLPCLTARD QDRLRATCTLSGNRDTLWHL FNTLQRRPGWVEYFIAALRG CELVDLADEVASVYQSYQPR TS

Data sets:
Data typeCount
13C chemical shifts466
15N chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAVS CARD1

Entities:

Entity 1, MAVS CARD 102 residues - Formula weight is not available

1   GLYSERMETPROPHEALAGLUASPLYSTHR
2   TYRLYSTYRILECYSARGASNPHESERASN
3   PHECYSASNVALASPVALVALGLUILELEU
4   PROTYRLEUPROCYSLEUTHRALAARGASP
5   GLNASPARGLEUARGALATHRCYSTHRLEU
6   SERGLYASNARGASPTHRLEUTRPHISLEU
7   PHEASNTHRLEUGLNARGARGPROGLYTRP
8   VALGLUTYRPHEILEALAALALEUARGGLY
9   CYSGLULEUVALASPLEUALAASPGLUVAL
10   ALASERVALTYRGLNSERTYRGLNPROARG
11   THRSER

Samples:

sample_1: MAVS CARD, [U-99% 13C; U-99% 15N], 20 mM; H2O, Natural Abundance, 100%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D DARRsample_1solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
3D CANCOsample_1solidsample_conditions_1

Software:

CcpNmr, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 25109
PDB
DBJ BAA86585 BAB14684 BAC77356 BAC85473 BAE79738
GB AAH44952 AAZ80417 ABA19229 ABA54890 ABR24162
REF NP_065797 XP_003821366 XP_008973040 XP_008973041 XP_009435017
SP Q7Z434