BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25034

Title: Amide 1H and 15N chemical shift assignments and 15N relaxation parameters of the troponin C-troponin I hybrid proteins cChimera   PubMed: 25010113

Authors: Pineda Sanabria, Sandra; Sykes, Brian; Julien, Olivier

Citation: Pineda-Sanabria, Sandra; Julien, Olivier; Sykes, Brian. "Versatile cardiac troponin chimera for muscle protein structural biology and drug discovery."  ACS Chem. Biol. 9, 2121-2130 (2014).

Assembly members:
cChimera, polymer, 141 residues, 15937 Da.
entity_CA, non-polymer, 40.078 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cChimera: MHHHHHHGGENLYFQGMDDI YKAAVEQLTEEQKNEFKAAF DIFVLGAEDGSISTKELGKV MRMLGQNPTPEELQEMIDEV DEDGSGTVDFDEFLVMMVRC MKDDSLVPPGSRRVRISADA MMQALLGARAKESLDLRAHL K

Data typeCount
1H chemical shifts183
15N chemical shifts95
heteronuclear NOE values183
T1 relaxation values181
T2 relaxation values182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cChimera1
2CALCIUM ION2

Entities:

Entity 1, cChimera 141 residues - 15937 Da.

Residues -15 to -8 correspond to the His tag. Residues -7 to -1 correspond to the TEV cleavage site. Residues 1 to 89 correspond to residues 1 to 89 of cNTnC. Residues 90 to 95 correspond to the thrombin cleavage site. Residues 144 to 173 correspond to 144 to 173 of cTnI.

1   METHISHISHISHISHISHISGLYGLYGLU
2   ASNLEUTYRPHEGLNGLYMETASPASPILE
3   TYRLYSALAALAVALGLUGLNLEUTHRGLU
4   GLUGLNLYSASNGLUPHELYSALAALAPHE
5   ASPILEPHEVALLEUGLYALAGLUASPGLY
6   SERILESERTHRLYSGLULEUGLYLYSVAL
7   METARGMETLEUGLYGLNASNPROTHRPRO
8   GLUGLULEUGLNGLUMETILEASPGLUVAL
9   ASPGLUASPGLYSERGLYTHRVALASPPHE
10   ASPGLUPHELEUVALMETMETVALARGCYS
11   METLYSASPASPSERLEUVALPROPROGLY
12   SERARGARGVALARGILESERALAASPALA
13   METMETGLNALALEULEUGLYALAARGALA
14   LYSGLUSERLEUASPLEUARGALAHISLEU
15   LYS

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: cChimera, [U-100% 15N], 0.5 – 0.8 mM; Ca2+ 2 mM; DTT 10 mM; potassium chloride 100 mM; imidazole 10 mM; D2O 5%

sample_conditions_1: temperature: 273 K; pH: 6.9; pressure: 1 atm; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView v8.2.33, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15385 15388 15400 15427 16190 16752 17103 19789 25120 25495 25797
PDB
DBJ BAA02369 BAG36483
EMBL CAA30736 CAG46663 CAG46683
GB AAA36772 AAA37492 AAA37493 AAA48654 AAB91994
PIR S07450 TPHUCC
PRF 1403394A 1510257A 750650A
REF NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_001291793
SP P02591 P05936 P09860 P19123 P63315
TPG DAA16908