BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25015

Title: holo_FldA_dynamics   PubMed: 25093851

Authors: Jin, Changwen; Ye, Qian; Hu, Yunfei

Citation: Ye, Qian; Hu, Yunfei; Jin, Changwen. "Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy."  PLoS ONE 9, e103936-e103936 (2014).

Assembly members:
entity, polymer, 176 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MAITGIFFGSDTGNTENIAK MIQKQLGKDVADVHDIAKSS KEDLEAYDILLLGIPTWYYG EAQCDWDDFFPTLEEIDFNG KLVALFGCGDQEDYAEYFCD ALGTIRDIIEPRGATIVGHW PTAGYHFEASKGLADDDHFV GLAIDEDRQPELTAERVEKW VKQISEELHLDEILNA

Data sets:
Data typeCount
heteronuclear NOE values163
T1 relaxation values163
T2 relaxation values163

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FldA1

Entities:

Entity 1, FldA 176 residues - Formula weight is not available

1   METALAILETHRGLYILEPHEPHEGLYSER
2   ASPTHRGLYASNTHRGLUASNILEALALYS
3   METILEGLNLYSGLNLEUGLYLYSASPVAL
4   ALAASPVALHISASPILEALALYSSERSER
5   LYSGLUASPLEUGLUALATYRASPILELEU
6   LEULEUGLYILEPROTHRTRPTYRTYRGLY
7   GLUALAGLNCYSASPTRPASPASPPHEPHE
8   PROTHRLEUGLUGLUILEASPPHEASNGLY
9   LYSLEUVALALALEUPHEGLYCYSGLYASP
10   GLNGLUASPTYRALAGLUTYRPHECYSASP
11   ALALEUGLYTHRILEARGASPILEILEGLU
12   PROARGGLYALATHRILEVALGLYHISTRP
13   PROTHRALAGLYTYRHISPHEGLUALASER
14   LYSGLYLEUALAASPASPASPHISPHEVAL
15   GLYLEUALAILEASPGLUASPARGGLNPRO
16   GLULEUTHRALAGLUARGVALGLULYSTRP
17   VALLYSGLNILESERGLUGLULEUHISLEU
18   ASPGLUILELEUASNALA

Samples:

sample_1: holo FldA, [U-15N], 1 mM; DTT 20 mM; sodium phosphate 30 mM; sodium chloride 30 mM; H2O 90%; D20 10%

sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
T1 measurementsample_1isotropicsample_conditions_1
T2 measurementsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 19945
PDB
DBJ BAA35333 BAB34138 BAG76270 BAI24076 BAI29544
EMBL CAD05157 CAP75173 CAQ31149 CAQ89923 CAQ97531
GB AAA23789 AAC73778 AAG55007 AAL19638 AAN79244
PIR AC0586
REF NP_308742 NP_415210 NP_455255 NP_459679 WP_000321745
SP P61949 P61950 P61951 Q8ZQX1