BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25014

Title: holo_YqcA_dynamics   PubMed: 25093851

Authors: Jin, Changwen; Hu, Yunfei; Ye, Qian

Citation: Ye, Qian; Hu, Yunfei; Jin, Changwen. "Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy."  PLoS ONE 9, e103936-e103936 (2014).

Assembly members:
YqcA, polymer, 149 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
YqcA: MAEIGIFVGTMYGNSLLVAE EAEAILTAQGHKATVFEDPE LSDWLPYQDKYVLVVTSTTG QGDLPDSIVPLFQGIKDSLG FQPNLRYGVIALGDSSYVNF CNGGKQFDALLQEQSAQRVG EMLLIDASENPEPETESNPW VEQWGTLLS

Data sets:
Data typeCount
heteronuclear NOE values135
T1 relaxation values136
T2 relaxation values136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YqcA1

Entities:

Entity 1, YqcA 149 residues - Formula weight is not available

1   METALAGLUILEGLYILEPHEVALGLYTHR
2   METTYRGLYASNSERLEULEUVALALAGLU
3   GLUALAGLUALAILELEUTHRALAGLNGLY
4   HISLYSALATHRVALPHEGLUASPPROGLU
5   LEUSERASPTRPLEUPROTYRGLNASPLYS
6   TYRVALLEUVALVALTHRSERTHRTHRGLY
7   GLNGLYASPLEUPROASPSERILEVALPRO
8   LEUPHEGLNGLYILELYSASPSERLEUGLY
9   PHEGLNPROASNLEUARGTYRGLYVALILE
10   ALALEUGLYASPSERSERTYRVALASNPHE
11   CYSASNGLYGLYLYSGLNPHEASPALALEU
12   LEUGLNGLUGLNSERALAGLNARGVALGLY
13   GLUMETLEULEUILEASPALASERGLUASN
14   PROGLUPROGLUTHRGLUSERASNPROTRP
15   VALGLUGLNTRPGLYTHRLEULEUSER

Samples:

sample_1: YqcA, [U-15N], 1 mM; DTT 20 mM; sodium phosphate 30 mM; sodium chloride 30 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
T1 measurementsample_1isotropicsample_conditions_1
T2 measurementsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18154 19151 19152 25013
PDB
DBJ BAB37073 BAE76862 BAG78574 BAI27051 BAI32080
EMBL CAP77223 CAQ33114 CAQ99718 CAR04300 CAR09403
GB AAB40440 AAC75832 AAG57904 AAN44291 AAN81803
REF NP_311677 NP_417270 NP_708584 WP_000229279 WP_000807726
SP P65367 P65368 P65369