BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20117

Title: Substance P 40 structures in water pH 5.5 298 K NMR DATA & STRUCTURES   PubMed: 20937248

Authors: Gayen, Anindita; Mukhopadhyay, Chaitali

Citation: Gayen, Anindita; Goswami, Sudipto Kishore; Mukhopadhyay, Chaitali. "NMR evidence of GM1-induced conformational change of Substance P using isotropic bicelles."  Biochim. Biophys. Acta 1808, 127-139 (2011).

Assembly members:
Substance_P, polymer, 11 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Substance_P: RPKPQQFFGLM

Data sets:
Data typeCount
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Substance P1

Entities:

Entity 1, Substance P 11 residues - Formula weight is not available

1   ARGPROLYSPROGLNGLNPHEPHEGLYLEU
2   MET

Samples:

Substance_P_in_DMPC_CHAPS_GM1_bicelles: Substance P 3.92 mM; DMPC 18.50 mM; CHAPS 74.00 mM; GM1 5.55 mM

DMPC_CHAPS_GM1_bicelles: pH: 5.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYSubstance_P_in_DMPC_CHAPS_GM1_bicellesisotropicDMPC_CHAPS_GM1_bicelles
2D 1H-1H NOESYSubstance_P_in_DMPC_CHAPS_GM1_bicellesisotropicDMPC_CHAPS_GM1_bicelles
2D DQF-COSYSubstance_P_in_DMPC_CHAPS_GM1_bicellesisotropicDMPC_CHAPS_GM1_bicelles

Software:

X-PLOR NIH, Charles Schwieters - structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz

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