BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 20074

Title: Protein Fibril   PubMed: 19130518

Authors: Nielsen, Jakob; Bjerring, Morten; Jeppesen, Martin; Pedersen, Ronnie; Pedersen, Jan; Hein, Kim; Vosegaard, Thomas; Skrydstrup, Troels; Otzen, Daniel; Nielsen, Niels Christian

Citation: Nielsen, Jakob; Bjerring, Morten; Jeppesen, Martin; Pedersen, Ronnie; Pedersen, Jan; Hein, Kim; Vosegaard, Thomas; Skrydstrup, Troels; Otzen, Daniel; Nielsen, Niels Christian. "Unique Identification of Supramolecular Structures in Amyloid Fibrils by Solid-State NMR Spectroscopy"  Angew. Chem. Int. Ed. Engl. 48, 2118-2121 (2009).

Assembly members:
NFGAILchain, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
NFGAILchain: SNNFGAILSS

Data sets:
Data typeCount
13C chemical shifts120
15N chemical shifts24

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit A1 peptide 11
2subunit A2 peptide 11
3subunit B1 peptide 11
4subunit B2 peptide 11
5subunit A1 peptide 21
6subunit A2 peptide 21
7subunit B1 peptide 21
8subunit B2 peptide 21

Entities:

Entity 1, subunit A1 peptide 1 10 residues - Formula weight is not available

NFGAILS is part of the decapeptide SNNFGAILSS, but structure data is only provided for the NFGAILS segment

1   SERASNASNPHEGLYALAILELEUSERSER

Samples:

SNNFGAILSS_U_13C_15N-_labeled_for_NFGAIL: NFGAIL, [U-100% 13C; U-100% 15N], 2.5 mg; H2O%; HEPES mM

sample_conditions_1: pH: 7.2; temperature: 263 K

Experiments:

NameSampleSample stateSample conditions
NCASNNFGAILSS_U_13C_15N-_labeled_for_NFGAILsolidsample_conditions_1
NCOSNNFGAILSS_U_13C_15N-_labeled_for_NFGAILsolidsample_conditions_1
2D 13C-13C DARR 2.5 ms mixingSNNFGAILSS_U_13C_15N-_labeled_for_NFGAILsolidsample_conditions_1
2D 13C-13C DARR 5 ms mixingSNNFGAILSS_U_13C_15N-_labeled_for_NFGAILsolidsample_conditions_1
2D 13C-13C DARR 20 ms mixingSNNFGAILSS_U_13C_15N-_labeled_for_NFGAILsolidsample_conditions_1
2D 13C-13C DARR 40 ms mixingSNNFGAILSS_U_13C_15N-_labeled_for_NFGAILsolidsample_conditions_1
2D 13C-13C DARR 100 ms mixingSNNFGAILSS_U_13C_15N-_labeled_for_NFGAILsolidsample_conditions_1
2D 13C-13C DARR 200 ms mixingSNNFGAILSS_U_13C_15N-_labeled_for_NFGAILsolidsample_conditions_1

Software:

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PIR P10997